BACKGROUND The present study on the role of strains of adenovirus in wildlife reservoirs, and their prevalence is under exploration. In several previous studies, the presence of adenovirus strains in wild birds has been investigated. Worldwide distribution and outbreaks of adenovirus infections have been reported by many authors. The present study investigated the prevalence of FAdVs in 317 samples of different bird species from the northwestern region of Poland. An applied specific, sensitive, and efficient, without cross-reactivity loop-mediated isothermal amplification (LAMP) method to gauge the prevalence of fowl adenovirus strains in wild birds was developed and used. RESULTS The method was based on the sequence of the loop L1 HVR1-4 region of the hexon gene of the FAdV genome reference strains FAdV-2 KT862805 (ANJ02325), FAdV-3 KT862807 (ANJ02399) and FAdV-11 KC750784 (AGK29904). The results obtained by LAMP were confirmed by real-time PCR. Among 317 samples obtained from wild birds, eight FAdV isolates (2.52%) were identified and produced a cytopathic effect (CPE) in chicken embryo kidney cells (CEK). Three FAdV types belonging to species Fowl adenovirus D were detected, which were isolated from three adenovirus types 2/3/11, and have been confirmed in three mute swans (Cygnus olor), three wild ducks (Anas platyrhynchos), one owl (Strigiformes), and one common wood pigeon (Columba palumbus). CONCLUSIONS This study provides the first accurate quantitative data for the replication of fowl adenovirus strains in wild birds in Poland, indicating adenovirus interspecies transmission, and demonstrating the circulation of FAdVs in wild birds.

中文翻译：

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通过环介导的等温扩增（LAMP）检测波兰野禽中的禽腺病毒D株。

背景技术目前关于腺病毒株在野生生物储库中的作用及其流行的研究正在探索中。在先前的一些研究中，已经研究了野禽中腺病毒株的存在。许多作者报道了腺病毒感染的全球分布和爆发。本研究调查了来自波兰西北部地区的317种鸟类鸟类中FAdV的流行情况。开发并使用了一种应用的，特异性，灵敏和有效的，无交叉反应的环介导的等温扩增（LAMP）方法来评估野禽中禽腺病毒株的流行情况。结果该方法基于FAdV基因组参考菌株FAdV-2 KT862805（ANJ02325）六邻体基因的L1环L1 HVR1-4区域的序列，FAdV-3 KT862807（ANJ02399）和FAdV-11 KC750784（AGK29904）。通过LAMP获得的结果通过实时PCR证实。在从野生鸟类获得的317个样本中，鉴定出8个FAdV分离株（2.52％），并在鸡胚肾细胞（CEK）中产生了细胞病变作用（CPE）。检测到三种FdV类型的禽F腺病毒，它们是从3/2/11三种腺病毒中分离出来的，并已在三只疣鼻天鹅（Cygnus olor），三只野鸭（Anas platyrhynchos），一只猫头鹰（Strigiformes）中得到证实。 ）和一只普通的木制鸽子（哥伦巴羽）。结论本研究为波兰野禽中家禽腺病毒株的复制提供了第一个准确的定量数据，表明腺病毒是种间传播的，并证明了FAdVs在野鸟中的循环。