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A preclinical large-animal model for the assessment of critical-size load-bearing bone defect reconstruction.
Nature Protocols ( IF 14.8 ) Pub Date : 2020-02-14 , DOI: 10.1038/s41596-019-0271-2
David S Sparks 1, 2, 3 , Siamak Saifzadeh 1, 4 , Flavia Medeiros Savi 1, 5 , Constantin E Dlaska 1, 6 , Arne Berner 1, 7 , Jan Henkel 1 , Johannes C Reichert 8, 9 , Martin Wullschleger 6, 10 , Jiongyu Ren 1 , Amaia Cipitria 11 , Jacqui A McGovern 1 , Roland Steck 4 , Michael Wagels 2, 3, 12 , Maria Ann Woodruff 5, 13 , Michael A Schuetz 1, 6 , Dietmar W Hutmacher 1, 5
Affiliation  

Critical-size bone defects, which require large-volume tissue reconstruction, remain a clinical challenge. Bone engineering has the potential to provide new treatment concepts, yet clinical translation requires anatomically and physiologically relevant preclinical models. The ovine critical-size long-bone defect model has been validated in numerous studies as a preclinical tool for evaluating both conventional and novel bone-engineering concepts. With sufficient training and experience in large-animal studies, it is a technically feasible procedure with a high level of reproducibility when appropriate preoperative and postoperative management protocols are followed. The model can be established by following a procedure that includes the following stages: (i) preoperative planning and preparation, (ii) the surgical approach, (iii) postoperative management, and (iv) postmortem analysis. Using this model, full results for peer-reviewed publication can be attained within 2 years. In this protocol, we comprehensively describe how to establish proficiency using the preclinical model for the evaluation of a range of bone defect reconstruction options.

中文翻译:

临床前大动物模型,用于评估临界尺寸的承重骨缺损重建。

需要大量组织重建的临界大小的骨缺损仍然是临床挑战。骨工程学有可能提供新的治疗概念,但是临床翻译需要解剖学和生理学相关的临床前模型。绵羊临界大小的长骨缺损模型已在众多研究中得到验证,可以作为评估常规和新型骨工程学概念的临床前工具。在大动物研究方面经过充分的培训和经验,当遵循适当的术前和术后管理规程时,这是技术上可行的方法,具有较高的可重复性。可以通过遵循以下步骤的程序来建立模型:(i)术前计划和准备,(ii)手术方法,(iii)术后管理,以及(iv)验尸分析。使用此模型,可以在2年内获得同行评审出版物的完整结果。在该协议中,我们全面描述了如何使用临床前模型来评估一系列骨缺损重建方案的能力。
更新日期:2020-02-14
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