The molecular organization of receptors in the plasma membrane of cells is paramount for their functionality. We combined lattice light-sheet (LLS) microscopy with three-dimensional (3D) single-molecule localization microscopy (dSTORM) and single-particle tracking to quantify the expression and distribution, and mobility of CD56 receptors on whole fixed and living cells, finding that CD56 accumulated at cell-cell interfaces. For comparison, we investigated two other receptors, CD2 and CD45, which showed different expression levels and distributions in the plasma membrane. Overall, 3D-LLS-dSTORM enabled imaging and single-particle tracking of plasma membrane receptors with single-molecule sensitivity unperturbed by surface effects. Our results demonstrate that receptor distribution and mobility are largely unaffected by contact to the coverslip but the measured localization densities are in general lower at the basal plasma membrane due to partial limited accessibility for antibodies.

中文翻译：

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通过3D格子光片dSTORM对质膜受体进行全细胞成像。

细胞质膜中受体的分子组织对其功能至关重要。我们将点阵光片（LLS）显微镜与三维（3D）单分子定位显微镜（dSTORM）和单颗粒跟踪相结合，以量化CD56受体在整个固定和活细胞上的表达和分布以及迁移率，从而发现CD56在单元间接口处累积。为了进行比较，我们研究了另外两个受体CD2和CD45，它们在质膜中显示出不同的表达水平和分布。总体而言，3D-LLS-dSTORM能够对质膜受体进行成像和单颗粒跟踪，且单分子灵敏度不受表面效应的干扰。