Abstract Berberis vulgaris (barberry) is a plant belonging to the Berberidaceae family and its medicinal properties have been known since ancient times. In this study, we have evaluated the antioxidant activity of fruit, leaves, root bark, and branch bark extracts of B. vulgaris, depending on the habitat and maturity by five methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity; 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation decolorization activity; total reducing power; cupric ion reducing antioxidant power; and the ferric ion reducing antioxidant power. Also, we determined the total phenolic and flavonoid contents. Of the analyzed extracts, the highest phenolic and flavonoid content was found in the extract of the fruit, 494 ± 2 µg GAE/mg dry extract weight and 2170 ± 4 µg RE/mg dry extract weight, respectively. Depending on the method used for the assay, the plant extracts show different antioxidant capacities but there is a certain pattern. All of the antioxidant assays have indicated the high antioxidant activity of B. vulgaris extracts. The extract of fruit stood out with the highest antioxidant activity while the bark of the roots of wild plants had the lowest activity. The study enhanced using chemometric analysis and cluster analysis was used to investigate the similarities between analyzed extracts.

中文翻译：

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小檗抗氧化特性的调查：化学和化学计量学方法

摘要 小檗（小檗）是小檗科植物，自古以来就知道其药用价值。在这项研究中，我们根据栖息地和成熟度，通过五种方法评估了 B. vulgaris 的果实、叶子、根皮和枝皮提取物的抗氧化活性： 2,2-diphenyl-1-picrylhydrazyl 自由基清除活性；2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)阳离子脱色活性；总还原力；铜离子还原抗氧化能力；和铁离子降低抗氧化能力。此外，我们还测定了总酚类和类黄酮含量。在分析的提取物中，果实提取物中的酚类和类黄酮含量最高，分别为 494 ± 2 µg GAE/mg 干提取物重量和 2170 ± 4 µg RE/mg 干提取物重量。根据用于测定的方法，植物提取物显示出不同的抗氧化能力，但存在一定的模式。所有的抗氧化试验都表明了 B. vulgaris 提取物的高抗氧化活性。水果提取物的抗氧化活性最高，而野生植物根皮的抗氧化活性最低。使用化学计量分析和聚类分析增强的研究用于研究分析提取物之间的相似性。水果提取物的抗氧化活性最高，而野生植物根皮的抗氧化活性最低。使用化学计量分析和聚类分析增强的研究用于研究分析提取物之间的相似性。水果提取物的抗氧化活性最高，而野生植物根皮的抗氧化活性最低。使用化学计量分析和聚类分析增强的研究用于研究分析提取物之间的相似性。