Abstract Here we present a novel strategy for the quantification of ascorbic acid (AA) (vitamin C). The developed method is based on the quenching effect produced by AA on the time-resolved luminescence signal of europium ions using excitation and emission wavelengths of 388 nm and 617 nm. The method presents a linear dynamic range from 0.2 to 2 µg mL−1 with a detection limit of 0.06 µg mL−1 and a relative standard deviation lower than 6%. The method was applied to the determination of AA in pharmaceutical preparations, human serum and human urine. The obtained results in pharmaceuticals were compared with a reference method; the recovery yields were close to 100% in all cases. The accuracy of the method was statistically assessed, demonstrating the suitability of this alternative method for the determination of AA in pharmaceuticals and biological samples, requiring no sample treatment except for a dilution step.

中文翻译：

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铕发光猝灭法测定药物和生物液体中的抗坏血酸

摘要 在这里，我们提出了一种量化抗坏血酸 (AA)（维生素 C）的新策略。开发的方法基于 AA 对铕离子的时间分辨发光信号产生的淬灭效应，使用 388 nm 和 617 nm 的激发和发射波长。该方法的线性动态范围为 0.2 至 2 µg mL-1，检测限为 0.06 µg mL-1，相对标准偏差低于 6%。该方法适用于药物制剂、人血清和人尿中AA的测定。将获得的药物结果与参考方法进行比较；在所有情况下，回收率都接近 100%。对方法的准确性进行了统计评估，