Flow-based biocatalysis offers advantages to perform multiphase reactions, including liquid–liquid reactions, due to intensified mass transfer, compartmentalization and high local concentration of the catalyst. Enzymatic immobilization leads to stable biocatalysts, with the possibility to incorporate them in continuous reactors. The combination between the two technologies allows for intensified process with high substrate concentration and high product recovery. The present paper is an excellent example of automated continuous biocatalytic process where a transferase from Mycobacterium smegmatis (MsAcT) was immobilized onto agarose beads and exploited for the preparation of a variety of flavour-esters, utilizing exclusively natural substrates, with excellent yields in 5-min reaction times. The corresponding products can be labelled and commercialized as natural too, thus increasing their market value.

Graphical abstract

基于流的生物催化由于加强了传质，分隔和催化剂的局部浓度高，因此具有进行多相反应（包括液-液反应）的优势。酶固定可产生稳定的生物催化剂，并有可能将其掺入连续反应器中。两种技术的结合可实现高底物浓度和高产品回收率的强化工艺。本文是自动化连续生物催化过程的一个很好的例子，其中耻垢分枝杆菌的转移酶将（MsAcT）固定在琼脂糖珠上，并利用纯天然底物在5分钟的反应时间内以极佳的收率用于制备各种风味酯。相应的产品也可以标记为天然产品并进行商品化，从而增加其市场价值。

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