This study evaluated the cardioprotective effects of a miR-1275 mimic in a rat model of myocardial ischemia-reperfusion (I/R)-induced myocardial injury (MI). Three groups of rats were established: a sham-operated group, a MI group and a MI+miR-1275 group pretreated for 1 week i.p. with a miR-1275 mimic at a concentration of 30 pmol/mL. MI was induced by I/R. The levels of myocardial enzymes in serum were estimated in all rats, together with haemodynamic functions. The effects of the miR-1275 mimic were determined based on the serum concentrations of inflammatory mediators in the treated vs. sham and MI rats. In addition, western blot assay and immunohistochemical analyses were performed to examine the effect of the miR-1275 mimic on the Wnt/NF-kB signalling pathway in MI rats. Treatment with the miR-1275 mimic attenuated the altered levels of myocardial enzymes and haemodynamic functions seen in MI rats. The myocardial infarct was smaller in rats treated with the miR-1275 mimic than in MI rats. The miR-1275 mimic also reduced myocardiocyte apoptosis and ameliorated the altered Wnt/KF-kB pathway. These results demonstrate the efficacy of the miR-1275 mimic in preventing myocardial I/R-induced MI in rats, by regulating the Wnt/NF-κB pathway.

中文翻译：

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miR-1275 模拟物通过调节心肌缺血再灌注诱导的心肌损伤大鼠模型中的 Wnt/NF-κB 通路来保护心肌细胞凋亡。

本研究评估了 miR-1275 模拟物在心肌缺血再灌注 (I/R) 诱导的心肌损伤 (MI) 大鼠模型中的心脏保护作用。建立了三组大鼠：假手术组、MI 组和 MI+miR-1275 组，用浓度为 30 pmol/mL 的 miR-1275 模拟物 ip 预处理 1 周。MI由I/R诱导。评估所有大鼠血清中心肌酶的水平，以及血流动力学功能。miR-1275 模拟物的作用是根据治疗组与假手术组和 MI 大鼠中炎症介质的血清浓度确定的。此外，还进行了蛋白质印迹分析和免疫组织化学分析，以检查 miR-1275 模拟物对 MI 大鼠 Wnt/NF-kB 信号通路的影响。用 miR-1275 模拟物治疗可减轻 MI 大鼠心肌酶水平和血流动力学功能的改变。与 MI 大鼠相比，用 miR-1275 模拟物治疗的大鼠的心肌梗塞更小。miR-1275 模拟物还减少了心肌细胞凋亡并改善了改变的 Wnt/KF-kB 通路。这些结果证明了 miR-1275 模拟物通过调节 Wnt/NF-κB 通路在预防大鼠心肌 I/R 诱导的 MI 方面的功效。