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Glucose, Cyc8p and Tup1p regulate biofilm formation and dispersal in wild Saccharomyces cerevisiae.
npj Biofilms and Microbiomes ( IF 9.2 ) Pub Date : 2020-02-13 , DOI: 10.1038/s41522-020-0118-1 Phu Van Nguyen 1 , Vítězslav Plocek 1 , Libuše Váchová 2 , Zdena Palková 1
npj Biofilms and Microbiomes ( IF 9.2 ) Pub Date : 2020-02-13 , DOI: 10.1038/s41522-020-0118-1 Phu Van Nguyen 1 , Vítězslav Plocek 1 , Libuše Váchová 2 , Zdena Palková 1
Affiliation
Saccharomyces cerevisiae is a mainly beneficial yeast, widely used in the food industry. However, there is growing evidence of its potential pathogenicity, leading to fungemia and invasive infections. The medical impact of yeast pathogens depends on formation of biofilms: multicellular structures, protected from the environment. Cell adhesion is a prerequisite of biofilm formation. We investigated the adherence of wild and genetically modified S. cerevisiae strains, formation of solid-liquid interface biofilms and associated regulation. Planktonic and static cells of wild strain BRF adhered and formed biofilms in glucose-free medium. Tup1p and Cyc8p were key positive and negative regulators, respectively. Glucose caused increased Cyc8p levels and blocked cell adhesion. Even low glucose levels, comparable with levels in the blood, allowed biofilm dispersal and release of planktonic cells. Cyc8p could thus modulate cell adhesion in different niches, dependently on environmental glucose level, e.g., high-glucose blood versus low-glucose tissues in host organisms.
中文翻译:
葡萄糖,Cyc8p和Tup1p调节野生酿酒酵母中生物膜的形成和扩散。
酿酒酵母是主要有益的酵母,广泛用于食品工业。但是,越来越多的证据表明其潜在的致病性,导致了真菌病和侵袭性感染。酵母菌病原体的医学影响取决于生物膜的形成:多细胞结构,不受环境影响。细胞粘附是生物膜形成的前提。我们调查了野生和基因改造啤酒酵母菌株的依从性,固液界面生物膜的形成和相关的监管。野生菌株BRF的浮游和静态细胞在无葡萄糖培养基中粘附并形成生物膜。Tup1p和Cyc8p分别是关键的正向和负向调节子。葡萄糖引起Cyc8p水平升高并阻止细胞粘附。即使是低血糖水平,也可以与血液中的血糖水平相比,允许生物膜扩散和释放浮游细胞。因此,Cyc8p可以根据环境葡萄糖水平,例如宿主生物体中的高血糖血液与低血糖组织,调节不同部位的细胞粘附。
更新日期:2020-02-13
中文翻译:
葡萄糖,Cyc8p和Tup1p调节野生酿酒酵母中生物膜的形成和扩散。
酿酒酵母是主要有益的酵母,广泛用于食品工业。但是,越来越多的证据表明其潜在的致病性,导致了真菌病和侵袭性感染。酵母菌病原体的医学影响取决于生物膜的形成:多细胞结构,不受环境影响。细胞粘附是生物膜形成的前提。我们调查了野生和基因改造啤酒酵母菌株的依从性,固液界面生物膜的形成和相关的监管。野生菌株BRF的浮游和静态细胞在无葡萄糖培养基中粘附并形成生物膜。Tup1p和Cyc8p分别是关键的正向和负向调节子。葡萄糖引起Cyc8p水平升高并阻止细胞粘附。即使是低血糖水平,也可以与血液中的血糖水平相比,允许生物膜扩散和释放浮游细胞。因此,Cyc8p可以根据环境葡萄糖水平,例如宿主生物体中的高血糖血液与低血糖组织,调节不同部位的细胞粘附。