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Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity.
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2020-02-12 , DOI: 10.1093/jxb/erz571
Bruno Pok Man Ngou 1 , Hee-Kyung Ahn 1 , Pingtao Ding 1 , Amey Redkar 1, 2 , Hannah Brown 1 , Yan Ma 1 , Mark Youles 1 , Laurence Tomlinson 1 , Jonathan D G Jones 1
Affiliation  

Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effectors AvrRps4 and PopP2, are well studied, but how the RRS1/RPS4 complex activates early immediate downstream responses upon effector detection is still poorly understood. To study RRS1/RPS4 responses without the influence of cell surface receptor immune pathways, we generated an Arabidopsis line with inducible expression of the effector AvrRps4. Induction does not lead to hypersensitive cell death response (HR) but can induce electrolyte leakage, which often correlates with plant cell death. Activation of RRS1 and RPS4 without pathogens cannot activate mitogen-associated protein kinase cascades, but still activates up-regulation of defence genes, and therefore resistance against bacteria.

中文翻译:

雌二醇诱导的 AvrRps4 表达揭示了 TIR-NLR 介导的效应触发免疫的不同特性。

植物核苷酸结合域、富含亮氨酸的重复受体 (NLR) 蛋白在识别病原体衍生的效应器中起重要作用。然而,植物NLRs激活免疫的机制仍然很大程度上未知。能够识别细菌效应物 AvrRps4 和 PopP2 的成对的拟南芥 NLRs RRS1-R 和 RPS4 得到了很好的研究,但 RRS1/RPS4 复合物如何在效应物检测时激活早期的直接下游反应仍然知之甚少。为了研究不受细胞表面受体免疫通路影响的 RRS1/RPS4 反应,我们生成了一个具有诱导型表达效应器 AvrRps4 的拟南芥系。诱导不会导致过敏性细胞死亡反应 (HR),但会导致电解质泄漏,这通常与植物细胞死亡有关。
更新日期:2020-03-26
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