Abstract

This study aims to produce a biodegradable active packaging film that is pH sensitive, and has a good antioxidant and antimicrobial activity. To do this, a novel phenolic extract was interfacially assembled onto a cellulose film, resulting in a film with a pH-triggered release mechanism of the active polyphenol agent. First, an aqueous extraction of cashew nut testa (CTE) was performed and subsequently, the CTE was characterized. The disc diffusion assay showed that CTE exhibited antimicrobial activity towards the food pathogens Escherichia coli (6 mm) and Staphylcoccus aureus (12 mm). CTE was also cytotoxic against cancer HepG2 and HEK293 cells, reducing the viability to 52% and 47%, respectively. It was incorporated into a cellulose-based packaging film, prepared from the by-product, sugarcane bagasse (SC) through interfacial assembly. The incorporation of CTE resulted in a film with good antimicrobial activity, excellent antioxidant content (91%), and has extremely high thermal stability (290 °C). FTIR indicated the formation of hydrogen bond between the SC cellulose-based film and CTE. The hydrogen bonds formed between the cellulose film and CTE became the driving force behind the pH-triggered release mechanism. It was found that the active agent, tannic acid, could be controllably released from the film, depending on the pH of the environment. Our strategy to produce a cellulose-based film impregnated with a phenolic extract, using interfacial assembly, resulted in an active packaging film with pH-triggered release mechanism. This film could be useful to extend the shelf life of perishable food items.

中文翻译：

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腰果（Anacardium occidentale）种皮提取物的界面组装到甘蔗渣中的纤维素基薄膜上，以产生具有pH触发释放机制的活性包装薄膜

摘要

这项研究的目的是生产一种对pH敏感的，具有良好的抗氧化和抗菌活性的可生物降解的活性包装膜。为此，将新的酚类提取物界面组装到纤维素膜上，形成具有pH触发的活性多酚剂释放机制的膜。首先，对腰果睾丸（CTE）进行水提取，然后对CTE进行表征。椎间盘扩散试验表明，CTE对食物病原体大肠杆菌（6毫米）和金黄色葡萄球菌具有抗菌活性。（12毫米）。CTE对癌细胞HepG2和HEK293也具有细胞毒性，将活力分别降低至52％和47％。它被掺入到纤维素基包装膜中，该膜由副产品甘蔗渣（SC）通过界面组装制得。掺入CTE的薄膜具有良好的抗菌活性，极好的抗氧化剂含量（91％），并具有极高的热稳定性（290°C）。FTIR表明SC纤维素基膜和CTE之间形成氢键。纤维素膜和CTE之间形成的氢键成为pH触发释放机制的驱动力。已经发现，取决于环境的pH，活性剂单宁酸可以从膜中可控地释放。我们采用界面组装的方法制备浸渍有酚类提取物的纤维素基薄膜，从而形成了一种具有pH触发释放机制的活性包装薄膜。这种薄膜可能有助于延长易腐食品的保质期。