BACKGROUND Bergeyella cardium infection is becoming increasingly prevalent in patients with infective endocarditis, suggesting its significance in disease pathogenesis. However, few studies have fully characterized this species. RESULTS Herein, we report the morphological and physiological characteristics, as well as whole genome sequencing of a newly identified B. cardium HPQL strain isolated from a patient with infective endocarditis. Results from the cellular morphology and biochemical analysis provide basic knowledge on the new pathogen. The whole genome sequencing of B. cardium HPQL consists of a circular chromosome with a total length of 2,036,890 bp. No plasmid was detected. Comparative genomics were carried out then. Antibiotics resistance related genes, pathogenesis related genes, predicted insertion sequences, genome islands and predicted CRISPRs sequences were demonstrated. To our knowledge, this is the first study to provide a complete genome sequence for Bergeyella spp. CONCLUSIONS This study provides fundamental phenotypic and genomic information for the newly identified fastidious infective endocarditis causative bacteria, B. cardium. Our results provide insights into effective clinical diagnosis and treatment of this pathogen.

中文翻译：

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一株与传染性心内膜炎有关的新型贝氏杆菌的鉴定和全基因组测序。

背景技术在感染性心内膜炎的患者中，伯氏杆菌的心脏感染正变得越来越普遍，表明其在疾病发病机理中的重要性。但是，很少有研究能够充分表征该物种。结果在此，我们报告了从感染性心内膜炎患者中分离出的一种新鉴定的B. Cardium HPQL菌株的形态和生理特性，以及全基因组测序。细胞形态学和生化分析的结果提供了有关新病原体的基础知识。芽孢杆菌HPQL的全基因组测序由一个环状染色体组成，总长度为2,036,890 bp。未检测到质粒。然后进行比较基因组学。抗生素抗性相关基因，发病机制相关基因，预测的插入序列，证明了基因组岛和预测的CRISPRs序列。据我们所知，这是第一项为伯氏杆菌属提供完整基因组序列的研究。结论本研究为新近鉴定的犬传染性心内膜炎致病细菌B. Cardium提供了基本的表型和基因组信息。我们的结果为对该病原体的有效临床诊断和治疗提供了见识。