Many skin diseases are defined by the presence of neutrophils, which are among the first cells to respond to infection and inflammation. Currently, neutrophil identification in the skin is costly and slow. The objectives of the present work are to investigate the feasibility of detecting the presence of neutrophils in live skin microsamples using chemiluminescence and develop a device and procedures that will enable preclinical and clinical investigations. Our approach consists of collecting skin microsamples and exposing them to reagents that activate neutrophils and amplify the light emission produced by chemiluminescence. Experiments using live pig skin with and without inflammation show that it is feasible to detect the presence of neutrophils in the skin. The proposed method is minimally invasive, simple, fast, and does not require user specialization. The developed system is compact in size with a small footprint, which makes it portable and suitable for point‐of‐care diagnostics.

中文翻译：

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使用化学发光法即时检测活体皮肤微量样品中的中性粒细胞。

中性粒细胞的存在是许多皮肤疾病的定义，中性粒细胞是对感染和炎症作出反应的首批细胞之一。当前，皮肤中嗜中性白细胞的鉴定昂贵且缓慢。本工作的目的是研究使用化学发光法检测活体皮肤微量样品中嗜中性粒细胞的存在的可行性，并开发一种能够进行临床前和临床研究的装置和程序。我们的方法包括收集皮肤微量样品，然后将它们暴露于激活嗜中性粒细胞并放大化学发光产生的光的试剂中。使用有或没有炎症的活猪皮肤进行的实验表明，检测皮肤中嗜中性粒细胞的存在是可行的。所提出的方法具有微创，简单，快速，并且不需要用户专门化。所开发的系统尺寸紧凑，占地面积小，因此便于携带，适用于即时诊断。