当前位置: X-MOL 学术Metabolomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative lipidomic analysis of inflammatory mediators in the aqueous humor and tear fluid of humans and rabbits.
Metabolomics ( IF 3.6 ) Pub Date : 2020-02-12 , DOI: 10.1007/s11306-020-1650-y
Dmitry V Chistyakov 1 , Nadezhda V Azbukina 1, 2 , Alina A Astakhova 1 , Sergei V Goriainov 3 , Viktor V Chistyakov 3 , Veronika V Tiulina 1 , Viktoriia E Baksheeva 1 , Vladislav I Kotelin 4 , Elena V Fedoseeva 4 , Andrey A Zamyatnin 1, 5 , Pavel P Philippov 1 , Olga A Kiseleva 4 , Alexander M Bessmertny 4 , Ivan I Senin 1 , Elena N Iomdina 4 , Marina G Sergeeva 1 , Evgeni Yu Zernii 1, 5
Affiliation  

INTRODUCTION Ocular inflammation is a key pathogenic factor in most blindness-causing visual disorders. It can manifest in the aqueous humor (AH) and tear fluid (TF) as alterations in polyunsaturated fatty acids (PUFAs) and their metabolites, oxylipins, lipid mediators, which are biosynthesized via enzymatic pathways involving lipoxygenase, cyclooxygenase or cytochrome P450 monooxygenase and specifically regulate inflammation and resolution pathways. OBJECTIVES This study aimed to establish the baseline patterns of PUFAs and oxylipins in AH and TF by their comprehensive lipidomic identification and profiling in humans in the absence of ocular inflammation and comparatively analyze these compounds in the eye liquids of rabbits, the species often employed in investigative ophthalmology. METHODS Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used for qualitative and quantitative characterization of lipid compounds in the analyzed samples. RESULTS A total of 28 lipid compounds were identified, including phospholipid derivatives and PUFAs, as well as 22 oxylipins. Whereas the PUFAs included arachidonic, docosahexaenoic and eicosapentaenoic acids, the oxylipins were derived mainly from arachidonic, linoleic and α-linolenic acids. Remarkably, although the concentration of oxylipins in AH was lower compared to TF, these liquids showed pronounced similarity in their lipid profiles, which additionally exhibited noticeable interspecies concordance. CONCLUSION The revealed correlations confirm the feasibility of rabbit models for investigating pathogenesis and trialing therapies of human eye disorders. The identified metabolite patterns suggest enzymatic mechanisms of oxylipin generation in AH and TF and might be used as a reference in ocular inflammation studies.

中文翻译:

人和兔子房水和泪液中炎性介质的比较脂质组学分析。

引言眼部炎症是大多数导致失明的视觉障碍的关键致病因素。它可以在房水(AH)和眼泪液(TF)中表现为多不饱和脂肪酸(PUFA)及其代谢产物,脂蛋白,脂质介体的改变,这些代谢物是通过涉及脂氧合酶,环氧合酶或细胞色素P450单加氧酶的酶促途径生物合成的。调节炎症和解决途径。目的本研究旨在通过在不存在眼部炎症的情况下对人进行全面脂质组学鉴定和分析,在AH和TF中建立PUFA和羟脂的基线模式,并在兔子的眼液中对这些化合物进行比较分析,这是经常用于研究的物种眼科。方法采用超高效液相色谱-串联质谱法(UPLC-MS / MS)对所分析样品中的脂质化合物进行定性和定量表征。结果共鉴定出28种脂质化合物,包括磷脂衍生物和PUFA,以及22种脂蛋白。尽管PUFA包括花生四烯酸,二十二碳六烯酸和二十碳五烯酸,但是脂蛋白主要衍生自花生四烯酸,亚油酸和α-亚麻酸。值得注意的是,虽然AH中的脂蛋白浓度低于TF,但这些液体的脂类特征显示出明显的相似性,此外还表现出明显的种间一致性。结论揭示的相关性证实了用于研究人眼疾病的发病机理和试验疗法的兔模型的可行性。
更新日期:2020-02-12
down
wechat
bug