Multifunctional tissue adhesives with excellent adhesion, antibleeding, anti-infection, and wound healing properties are desperately needed in clinical surgery. However, the successful development of multifunctional tissue adhesives that simultaneously possess all these properties remains a challenge. We have prepared a novel chitosan-based hydrogel adhesive by integration of hydrocaffeic acid-modified chitosan (CS-HA) with hydrophobically modified chitosan lactate (hmCS lactate) and characterized its gelation time, mechanical properties, and microstructure. Tissue adhesion properties were evaluated using both pigskin and intestine models. In situ antibleeding efficacy was demonstrated via the rat hemorrhaging liver and full-thickness wound closure models. Good antibacterial activity and anti-infection capability toward S. aureus and P. aeruginosa were confirmed using in vitro contact-killing assays and an infected pigskin model. The result of coculturing with 3T3 fibroblast cells indicated that the hydrogels have no significant cytotoxicity. Most importantly, the biocompatible and biodegradable CS-HA/hmCS lactate hydrogel was able to close the wound in a sutureless way and promote wound healing. Our results demonstrate that this hydrogel has great promise for sutureless closure of surgical incisions.

中文翻译：

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抗感染和促凝壳聚糖基水凝胶组织粘合剂，可无创缝合。

急需临床手术中具有优异粘附力，抗出血，抗感染和伤口愈合特性的多功能组织粘合剂。然而，同时具有所有这些特性的多功能组织粘合剂的成功开发仍然是一个挑战。我们通过将咖啡因酸改性的壳聚糖（CS-HA）与疏水改性的壳聚糖乳酸酯（hmCS乳酸酯）集成在一起，制备了一种新型的壳聚糖基水凝胶粘合剂，并表征了其胶凝时间，机械性能和微观结构。使用猪皮和肠模型评估组织粘附特性。通过大鼠出血性肝和全层伤口闭合模型证明了原位抗出血功效。对金黄色葡萄球菌和假单胞菌具有良好的抗菌活性和抗感染能力。使用体外接触杀伤试验和感染的猪皮模型确认了铜绿假单胞菌。与3T3成纤维细胞共培养的结果表明，水凝胶没有明显的细胞毒性。最重要的是，具有生物相容性且可生物降解的CS-HA / hmCS乳酸水凝胶能够以无缝合的方式闭合伤口并促进伤口愈合。我们的结果表明，这种水凝胶有望实现无创缝合手术切口。