As synthetic biology and metabolic engineering tools improve, it is feasible to construct more complex microbial synthesis systems that may be limited by the machinery and resources available in an individual cell. Coculture fermentation is a promising strategy for overcoming these constraints by distributing objectives between subpopulations, but the primary method for controlling the composition of the coculture of production systems has been limited to control of the inoculum composition. We have developed a quorum sensing (QS)-based growth-regulation circuit that provides an additional parameter for regulating the composition of a coculture over the course of the fermentation. Implementation of this tool in a naringenin-producing coculture resulted in a 60% titer increase over a system that was optimized by varying inoculation ratios only. We additionally demonstrated that the growth control circuit can be implemented in combination with a communication module that couples transcription in one subpopulation to the cell-density of the other population for coordination of behavior, resulting in an additional 60% improvement in naringenin titer.

中文翻译：

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开发一种基于群体感应的控制系统，用于控制柚皮素生产系统中的共培养种群组成。

随着合成生物学和代谢工程工具的改进，构建更复杂的微生物合成系统是可行的，该系统可能受到单个细胞中可用的机械和资源的限制。共培养发酵是通过在亚群之间分配目标来克服这些限制的有前途的策略，但是控制生产系统共培养的组成的主要方法仅限于控制接种物的组成。我们已经开发了基于群体感应（QS）的生长调节电路，该电路提供了用于在发酵过程中调节共培养物组成的其他参数。在产生柚皮素的共培养物中实施该工具可使系统的滴度提高，而该系统仅通过改变接种比例即可优化。