Tandem mass spectrometry involves isolation of specific precursor ions and their subsequent excitation through collision‐, photon‐, or electron‐mediated activation techniques in order to induce unimolecular dissociation leading to formation of fragment ions. These powerful ion activation techniques, typically used in between mass selection and mass analysis steps for structural elucidation, have not only found a wide variety of analytical applications in chemistry and biology, but they have also been used to study the fundamental properties of ions in the gas phase. In this tutorial paper, a brief overview is presented of the theories that have been used to describe the activation of ions and their subsequent unimolecular dissociation. Acronyms of the presented techniques include CID, PQD, HCD, SORI, SID, BIRD, IRMPD, UVPD, EPD, ECD, EDD, ETD, and EID. The fundamental principles of these techniques are discussed in the context of their implementation on ultra‐high resolution tandem mass spectrometers. © 2020 John Wiley & Sons Ltd. Mass Spec Rev

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教程：使用超高分辨率仪器在串联质谱中进行离子活化。

串联质谱法涉及特定前体离子的分离及其随后通过碰撞，光子或电子介导的活化技术的激发，以诱导单分子解离，从而导致碎片离子的形成。这些功能强大的离子活化技术通常用于质量选择和质量分析步骤之间，以阐明结构，不仅在化学和生物学领域发现了广泛的分析应用，而且还被用于研究离子在色谱柱中的基本性质。气相。在本教程文件中，将简要概述已用于描述离子激活及其随后的单分子解离的理论。所提供技术的首字母缩写包括CID，PQD，HCD，SORI，SID，BIRD，IRMPD，UVPD，EPD，ECD，EDD，ETD，和EID。这些技术的基本原理将在超高分辨率串联质谱仪上的实施中进行讨论。©2020 John Wiley＆Sons Ltd.质谱修订版