Rhodococcus rhodochrous ATCC 21198 (strain ATCC 21198) was successfully co-encapsulated in gellan gum beads with orthosilicates as slow release compounds (SRCs) to support aerobic cometabolism of a mixture of 1,1,1-trichloroethane (1,1,1-TCA), cis-1,2-dichloroethene (cis-DCE), and 1,4-dioxane (1,4-D) at aqueous concentrations ranging from 250 to 1000 μg L-1. Oxygen (O2) consumption and carbon dioxide (CO2) production showed the co-encapsulated cells utilized the alcohols that were released from the co-encapsulated SRCs. Two model SRCs, tetrabutylorthosilicate (TBOS) and tetra-s-butylorthosilicate (T2BOS), which hydrolyze to produce 1- and 2-butanol, respectively, were encapsulated in gellan gum (GG) at mass loadings as high as 10% (w/w), along with strain ATCC 21198. In the GG encapsulated beads, TBOS hydrolyzed 26 times faster than T2BOS and rates were ∼4 times higher in suspension than when encapsulated. In biologically active reactors, the co-encapsulated strain ATCC 21198 effectively utilized the SRC hydrolysis products (1- and 2-butanol) and cometabolized repeated additions of a mixture of 1,1,1-TCA, cis-DCE, and 1,4-D for over 300 days. The transformation followed pseudo-first-order kinetics. Vinyl chloride (VC) and 1,1-dichloroethene (1,1-DCE) were also transformed in the reactors after 250 days. In the long-term treatment, the batch reactors with co-encapsulated T2BOS GG beads achieved similar transformation rates, but at much lower O2 consumption rates than those with TBOS. The results demonstrate that the co-encapsulation technology can be a passive method for the cometabolic treatment of dilute groundwater plumes.

中文翻译：

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缓释化合物和Rhodococcus rhodchrous ATCC 21198在结冷胶珠中的共包封可促进1,1,1-三氯乙烷，顺式1,2-二氯乙烯和1,4-二恶烷的长期有氧代谢性转化。

杜鹃红球菌ATCC 21198（ATCC 21198菌株）与原硅酸盐成功地共包封在吉兰糖胶珠中，作为缓释化合物（SRC），以支持1,1,1-三氯乙烷（1,1,1-TCA）混合物的有氧代谢），顺式1,2-二氯乙烯（cis-DCE）和1,4-二恶烷（1,4-D）的水溶液浓度范围为250至1000μgL-1。氧气（O2）的消耗和二氧化碳（CO2）的产生表明，共包封的细胞利用了从共包封的SRC中释放出的醇。两种水解型SRC，分别水解生成1-丁醇和2-丁醇的原硅酸四丁酯（TBOS）和原硅酸四丁酯（T2BOS），以高达10％（w / w）的质量负载封装在吉兰糖胶（GG）中w），以及ATCC 21198菌株。在GG包封的磁珠中，TBOS的水解速度比T2BOS快26倍，悬浮速率比封装时高约4倍。在具有生物活性的反应器中，共包封的菌株ATCC 21198有效利用了SRC水解产物（1-丁醇和2-丁醇），并重复代谢了1,1,1-TCA，顺式DCE和1,4的混合物-D超过300天。该转变遵循伪一级动力学。250天后，氯乙烯（VC）和1,1-二氯乙烯（1,1-DCE）也在反应器中转化。在长期处理中，具有共包封的T2BOS GG珠的间歇式反应器实现了相似的转化率，但与TBOS相比，其O2消耗率低得多。结果表明，共包封技术可以作为一种被动方法，用于淡水羽流的新陈代谢处理。