BACKGROUND This study aimed to explore the diagnostic value of serum miR-101-3p combined with pepsinogen (PG) on early diagnosis of gastric cancer (GC). METHODS A total of 61 atrophic gastritis (AG) and 86 GC patients, and 50 healthy volunteers were enrolled. The serum expression of miR-101-3p was measured by qRT-PCR. The serum content of carcinoembryonic antigen (CEA) was measured by Electrochemiluminescence immunoassay. The serum contents of PGI and PGII were measured by Enzyme linked immunosorbent assay. The diagnostic value of serum markers on AG and GC was analyzed by receiver operating characteristic (ROC) analysis. RESULTS The expression of miR-101-3p, the content of PGI and the ratio of PGI/II were significantly decreased, and the content of PGII was significantly increased in AG patients compared with those in normal controls. The changes of the above serum indicators were more obvious in GC patients than those in AG patients. The content of CEA was significantly higher in GC patients than that in AG patients. In addition, the expression of miR-101-3p was negatively associated with the submucosal infiltration in GC patients. MiR-101-3p exhibited high diagnostic value on AG (AUC 0.8493, sensitivity 80.33%, specificity 80%) and GC (AUC 0.8749, sensitivity 72.09%, specificity 86.49%). MiR-101-3p + PGI + PGI/II (AUC 0.856, sensitivity 80.23%, specificity 77.05%) exhibited a high diagnostic value in distinguishing between AG and GC. CONCLUSIONS MiR-101-3p was a potential diagnostic marker for AG and GC. MiR-101-3p + PGI + PGI/II was effective in distinguishing between AG and GC.

中文翻译：

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血清miR-101-3p结合胃蛋白酶原有助于早期诊断胃癌。

背景技术本研究旨在探讨血清miR-101-3p结合胃蛋白酶原（PG）在胃癌（GC）早期诊断中的诊断价值。方法共有61例萎缩性胃炎（AG）和86例GC患者，以及50名健康志愿者。通过qRT-PCR测量miR-101-3p的血清表达。通过电化学发光免疫测定法测定血清癌胚抗原（CEA）。通过酶联免疫吸附法测定PGI和PGII的血清含量。血清标志物对AG和GC的诊断价值通过接受者操作特征（ROC）分析进行分析。结果与正常对照组相比，AG患者的miR-101-3p的表达，PGI的含量和PGI / II的比例均明显降低，而PGII的含量则明显升高。上述血清指标的变化在GC患者中比在AG患者中更明显。GC患者的CEA含量明显高于AG患者。此外，miR-101-3p的表达与GC患者的粘膜下浸润负相关。MiR-101-3p对AG（AUC 0.8493，灵敏度80.33％，特异性80％）和GC（AUC 0.8749，灵敏度72.09％，特异性86.49％）表现出较高的诊断价值。MiR-101-3p + PGI + PGI / II（AUC 0.856，灵敏度80.23％，特异性77.05％）在区分AG和GC方面具有很高的诊断价值。结论MiR-101-3p是AG和GC的潜在诊断标记。MiR-101-3p + PGI + PGI / II可有效区分AG和GC。GC患者的CEA含量明显高于AG患者。此外，miR-101-3p的表达与GC患者的粘膜下浸润负相关。MiR-101-3p对AG（AUC 0.8493，灵敏度80.33％，特异性80％）和GC（AUC 0.8749，灵敏度72.09％，特异性86.49％）表现出较高的诊断价值。MiR-101-3p + PGI + PGI / II（AUC 0.856，灵敏度80.23％，特异性77.05％）在区分AG和GC方面具有很高的诊断价值。结论MiR-101-3p是AG和GC的潜在诊断标记。MiR-101-3p + PGI + PGI / II可有效区分AG和GC。GC患者的CEA含量明显高于AG患者。此外，miR-101-3p的表达与GC患者的粘膜下浸润负相关。MiR-101-3p对AG（AUC 0.8493，灵敏度80.33％，特异性80％）和GC（AUC 0.8749，灵敏度72.09％，特异性86.49％）表现出较高的诊断价值。MiR-101-3p + PGI + PGI / II（AUC 0.856，灵敏度80.23％，特异性77.05％）在区分AG和GC方面具有很高的诊断价值。结论MiR-101-3p是AG和GC的潜在诊断标记。MiR-101-3p + PGI + PGI / II可有效区分AG和GC。MiR-101-3p对AG（AUC 0.8493，灵敏度80.33％，特异性80％）和GC（AUC 0.8749，灵敏度72.09％，特异性86.49％）表现出较高的诊断价值。MiR-101-3p + PGI + PGI / II（AUC 0.856，灵敏度80.23％，特异性77.05％）在区分AG和GC方面具有很高的诊断价值。结论MiR-101-3p是AG和GC的潜在诊断标记。MiR-101-3p + PGI + PGI / II可有效区分AG和GC。MiR-101-3p对AG（AUC 0.8493，灵敏度80.33％，特异性80％）和GC（AUC 0.8749，灵敏度72.09％，特异性86.49％）表现出较高的诊断价值。MiR-101-3p + PGI + PGI / II（AUC 0.856，灵敏度80.23％，特异性77.05％）在区分AG和GC方面具有很高的诊断价值。结论MiR-101-3p是AG和GC的潜在诊断标记。MiR-101-3p + PGI + PGI / II可有效区分AG和GC。