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A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper.
BMC Genomics ( IF 4.4 ) Pub Date : 2020-02-10 , DOI: 10.1186/s12864-020-6556-6
Jiaoyan Tan 1 , Yan Wu 1, 2 , Jianping Guo 1 , Huimin Li 1 , Lili Zhu 1 , Rongzhi Chen 1 , Guangcun He 1 , Bo Du 1
Affiliation  

BACKGROUND The brown planthopper (BPH, Nilaparvata lugens Stål) is a kind of phloem-feeding pest that adversely affects rice yield. Recently, the BPH-resistance gene, BPH6, was cloned and applied in rice breeding to effectively control BPH. However, the molecular mechanisms underlying BPH6 are poorly understood. RESULTS Here, an integrated miRNA and mRNA expression profiling analysis was performed on BPH6-transgenic (BPH6G) and Nipponbare (wild type, WT) plants after BPH infestation, and a total of 217 differentially expressed miRNAs (DEMs) and 7874 differentially expressed mRNAs (DEGs) were identified. 29 miRNAs, including members of miR160, miR166 and miR169 family were opposite expressed during early or late feeding stages between the two varieties, whilst 9 miRNAs were specifically expressed in BPH6G plants, suggesting involvement of these miRNAs in BPH6-mediated resistance to BPH. In the transcriptome analysis, 949 DEGs were opposite expressed during early or late feeding stages of the two genotypes, which were enriched in metabolic processes, cellular development, cell wall organization, cellular component movement and hormone transport, and certain primary and secondary metabolite synthesis. 24 genes were further selected as candidates for BPH resistance. Integrated analysis of the DEMs and DEGs showed that 34 miRNAs corresponding to 42 target genes were candidate miRNA-mRNA pairs for BPH resistance, 18 pairs were verified by qRT-PCR, and two pairs were confirmed by in vivo analysis. CONCLUSIONS For the first time, we reported integrated small RNA and transcriptome sequencing to illustrate resistance mechanisms against BPH in rice. Our results provide a valuable resource to ascertain changes in BPH-induced miRNA and mRNA expression profiles and enable to comprehend plant-insect interactions and find a way for efficient insect control.

中文翻译:

结合的microRNA和转录组分析阐明了水稻对褐飞虱的抗性响应。

背景技术褐飞虱(BPH,Nilaparvata lugensStål)是一种韧皮部害虫,对水稻的产量产生不利影响。最近,克隆了抗BPH的基因BPH6,并将其用于水稻育种以有效控制BPH。但是,对BPH6的分子机制了解甚少。结果在BPH侵染后,对BPH6转基因(BPH6G)和Nipponbare(野生型,WT)植物进行了综合的miRNA和mRNA表达谱分析,共有217个差异表达的miRNA(DEM)和7874个差异表达的mRNA( DEGs)。在两个变种的早期或晚期进食阶段,包括miR160,miR166和miR169家族成员在内的29种miRNA相反表达,而9种miRNA在BPH6G植物中特异性表达,提示这些miRNA参与了BPH6介导的BPH抗性。在转录组分析中,在两种基因型的早期或晚期进食阶段,有949个DEG表达相反,它们在代谢过程,细胞发育,细胞壁组织,细胞成分移动和激素转运以及某些初级和次级代谢产物的合成中富集。进一步选择了24个基因作为BPH抗性的候选基因。对DEM和DEG的综合分析表明,与42个靶基因相对应的34个miRNA是BPH抗性的候选miRNA-mRNA对,通过qRT-PCR验证了18对,在体内分析中证实了2对。结论我们首次报道了整合的小RNA和转录组测序,以阐明水稻对BPH的抗性机制。
更新日期:2020-02-10
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