Hydrophobic agents, a fluorescent dye (Nile red, NR) or an anticancer drug (doxorubicin, DOX), are encapsulated into poly((N‐[3‐(dimethylamino) propyl] methacrylamide)‐b‐poly (methyl methacrylate) (PDMAPMA‐b‐PMMA) nanoparticles (NPs) via one‐pot reversible addition‐fragmentation chain‐transfer (RAFT)‐mediated emulsion polymerization in water. The macroRAFT, PDMAPMA, is chain‐extended with the methyl methacrylate (MMA), with the hydrophobic agents soluble in MMA, resulting in loaded NPs, with either NR or DOX via polymerization‐induced self‐assembly (PISA). The NR‐loaded NPs are visualized by structured illumination microscopy (SIM), thus indicating the successful loading of the fluorescent dye into the PMMA core. The DOX‐loaded NPs exhibit a sustained release profile over 5 d, showing a small burst effect during the first 2 h, as compared with the free DOX. The DOX‐loaded NPs show higher cell toxicity than the free DOX in RAW 264.7 cell line. The results demonstrate the potential of using emulsion polymerization for synthesis of tailored and reproducible NPs encapsulating hydrophobic agents.

中文翻译：

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尼罗红或阿霉素在RAFT介导的乳液聚合过程中通过聚合诱导的自组装在生物医学应用中原位包封

疏水剂，荧光染料（尼罗红（Nile red），NR）或抗癌药（阿霉素（Doxorubicin，DOX））封装在聚（（N- [3-（二甲基氨基）丙基]甲基丙烯酰胺）b-聚甲基丙烯酸甲酯（PDMAPMA）中‐ b-PMMA）纳米粒子（NPs）通过一锅可逆加成-断裂链转移（RAFT）介导的乳液聚合在水中进行。macroRAFT PDMAPMA与甲基丙烯酸甲酯（MMA）进行链延长，疏水剂可溶于MMA，通过聚合诱导的自组装（PISA）与NR或DOX形成负载的NP。通过结构化照明显微镜（SIM）可视化NR负载的NP，从而表明荧光染料已成功加载到PMMA核中。负载DOX的NPs在5 d内呈现出持续释放的特征，与游离DOX相比，在头2 h内表现出较小的爆发效应。载有DOX的NP比RAW 264.7细胞系中的游离DOX具有更高的细胞毒性。