Electrolytic Electroporation (E2) is the combination of reversible electroporation and electrolysis. It has been proposed as a novel treatment option to ablate tissue percutaneously. The present in vitro study in cells in suspension was performed to investigate the underlying mechanisms of action of E2. Different types of experiments were performed to isolate the effects of the electrolysis and the electroporation components of the treatment. Additionally, thermal simulations were performed to determine whether significant temperature increase contributes to the effect. The results indicate that E2's cell killing efficacy is due to a combinational effect of electrolysis and reversible electroporation that takes place within the first two minutes after E2 application. The results further show that cell death after E2 treatment is significantly delayed. These observations suggest that cell death is induced in permeabilized cells due to the uptake of electrolysis species. Thermal simulations revealed a significant but innocuous temperature increase.

中文翻译：

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电解电穿孔（E2）作用机理的体外研究。

电解电穿孔（E2）是可逆电穿孔和电解的组合。已经提出将其作为经皮消融组织的新的治疗选择。目前在悬浮细胞中的体外研究是为了研究E2作用的潜在机制。进行不同类型的实验以分离处理的电解作用和电穿孔组分。另外，进行了热模拟以确定温度的显着升高是否对该效应有所贡献。结果表明，E2的细胞杀伤功效归因于电解和可逆电穿孔的组合作用，这种作用发生在E2施用后的前两分钟之内。结果进一步表明，E2处理后细胞死亡显着延迟。这些观察结果表明，由于吸收了电解物质，因此在透化细胞中诱导了细胞死亡。热模拟显示出明显但无害的温度升高。