Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2-3 h (40-50% faster than conventional approaches) with <0.5 mL of reagents, signifying that this device could serve as a promising diagnostic tool for ovarian cancer.

中文翻译：

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集成的微流控系统，用于对临床组织样本进行快速，自动和高通量染色以诊断卵巢癌。

迫切需要准确的癌症诊断方法。由于传统的基于免疫组织化学（IHC）的方法虽然可靠，但劳动强度大且需要训练有素的技术人员，所以我们开发了一种集成的微流体平台，该平台能够通过分子标记福尔马林固定的石蜡包埋组织中的卵巢癌生物标记物（即适体）探针。基于适配子的1）荧光染色和2）临床组织样品的IHC染色都可以在微流体系统中仅用2-3小时（比传统方法快40-50％），并使用<0.5 mL试剂自动进行，这表明该设备可以作为卵巢癌的有前途的诊断工具。