AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted attention due to their high sensitivity, stability, and fast response characteristics. Some related studies have been explored but a Debye screening problem exists in physiological solutions hindering the detection of bio-macromolecules. Herein, a novel fast analytical platform for electronic enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the sensing surface to simplify the assay. Combining the advantages of e-ELISA and MBs, the resulting analytical platform presents a sensing capability beyond the Debye-screening limit and a novel ability to be reused. This platform offers a fast response toward prostate specific antigen (PSA) and the lowest concentration of detection is 1 fg mL-1. Compared with conventional AlGaN/GaN HEMT biosensors, it shows higher sensitivity (3.73 μA dec-1) in a linear range (1 fg mL-1 to 1 pg mL-1), which is within the constraints of emergency care applications. The platform's high sensitivity and fast repeatability endow it with great potential for early and rapid diagnosis.

中文翻译：

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电子酶联免疫吸附测定平台，用于基于磁珠和AlGaN / GaN高电子迁移率晶体管的蛋白质分析。

AlGaN / GaN高电子迁移率晶体管（HEMT）生物传感器因其高灵敏度，稳定性和快速响应特性而备受关注。已经探索了一些相关的研究，但是在生理溶液中存在德拜（Debye）筛选问题，阻碍了生物大分子的检测。本文提出了一种基于AlGaN / GaN HEMT和磁珠的新型快速分析平台，用于电子酶联免疫吸附测定（e-ELISA）。基于MB的e-ELISA将修饰区域与传感表面解耦，从而简化了测定过程。结合了e-ELISA和MB的优势，最终的分析平台提供了超越Debye筛选限制的传感能力和可重复使用的新颖能力。该平台可快速响应前列腺特异性抗原（PSA），最低检测浓度为1 fg mL-1。与传统的AlGaN / GaN HEMT生物传感器相比，它在线性范围（1 fg mL-1至1 pg mL-1）中显示出更高的灵敏度（3.73μAdec-1），这在紧急护理应用的限制之内。该平台的高灵敏度和快速可重复性使其具有早期和快速诊断的巨大潜力。