Pb2+ is a xenobiotic metal ion that competes for Ca2+-binding sites in proteins. Using the peripheral Ca2+-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb2+ sites that are likely to be targeted by bioavailable Pb2+.

中文翻译：

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pH缓冲液对Pb2 +-外围结构域相互作用的干扰：障碍还是机遇？

Pb2 +是一种异源金属离子，可竞争蛋白质中的Ca2 +结合位点。使用Syt1的外围Ca2 +感应域，我们显示螯合的pH缓冲液Bis-Tris可以识别和亲和力高生物亲和性Pb2 +可能靶向的高亲和力Pb2 +位点。