A novel and simple interface used in a high-performance liquid chromatography-catalytic cold vapor-atomic fluorescence spectrometry (HPLC-CCV-AFS) hyphenated system without post-column digestion, based on the catalytic degradation of organic mercury by directly introducing Fe³⁺ into the carrier, was developed for the speciation of mercury. The synergy of Fe³⁺ with L-cysteine or mercaptoethanol reduces the Fe³⁺ concentration. The parameters influencing mercury separation and determination were optimized, including the content of Fe³⁺ in the carrier, the concentration of L-cysteine, KBH₄ and HCl, and the flow rate of the carrier gas and shielding gas. Under the optimized conditions, the limits of detection (LODs) for monomethylmercury (MeHg), ethylmercury (EtHg) and Hg²⁺ (IHg), based on a 100 μL sample, were 0.06, 0.09, and 0.13 μg L⁻¹, while the LODs of MeHg, EtHg and IHg in food samples, based on 0.2 g sample and 50 mL final sample volume, were 15, 23 and 33 μg kg⁻¹, respectively. The proposed method with a simple interface, high sensitivity, low instrument and operation cost, easy operation, reduced damage to the instrument pipeline and reduced use of harmful and organic reagents was successfully applied to fish samples and is suitable for routine use.

中文翻译：

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Fe3 +催化降解有机汞，作为高效液相色谱-催化冷蒸气-原子荧光光谱法测定汞形态的简单柱后界面

通过直接引入Fe ³⁺催化降解有机汞，在无需柱后消解的高效液相色谱-催化冷气相原子荧光光谱法（HPLC-CCV-AFS）联用系统中使用新颖新颖的界面进入载体，被开发用于汞的形成。Fe ³⁺与L-半胱氨酸或巯基乙醇的协同作用降低了Fe ^3+的浓度。优化了影响汞分离和测定的参数，包括载体中Fe ³⁺的含量，L-半胱氨酸的浓度，KBH ₄HCl，载气和保护气的流量。在最佳条件下，基于100μL样品，单甲基汞（MeHg），乙基汞（EtHg）和Hg ²⁺（IHg）的检出限（LOD）为0.06、0.09和0.13μgL ^-1，而基于0.2 g样品和50 mL最终样品量，食品样品中MeHg，EtHg和IHg的LOD分别为15、23和33μgkg ^-1。所提出的方法界面简单，灵敏度高，仪器和操作成本低，易于操作，减少了仪器管线的损坏，减少了有害和有机试剂的使用，已成功应用于鱼类样品，适合常规使用。