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Spectral quality as an elicitor of bioactive compound production in Solanum aculeatissimum JACQ cell suspension.
Journal of Photochemistry and Photobiology B: Biology ( IF 5.4 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.jphotobiol.2020.111819
Luciana Arantes Dantas 1 , Márcio Rosa 1 , Erika Crispim Resende 2 , Fabiano Guimarães Silva 3 , Paulo Sérgio Pereira 4 , Ana Cristina Lourenço Souza 3 , Fernando Higino de Lima E Silva 3 , Aurélio Rubio Neto 3
Affiliation  

Solanum aculeatissimum Jacq. is a common plant in much of Brazil. Despite containing metabolites with a wide range of pharmacological applications, there are few tissue culture reports for this plant. The possibility of large-scale in vitro production of this material has significant biotechnological potential. Therefore, the objective of this study was to investigate the effect of light conditions on the growth of cells in suspension, observing the production and yield of biomass and bioactive compounds and the enzymatic behavior. Calli obtained from leaf segments were cultured in solid medium supplemented with 1 mg L-1 of 2,4-D, 2.5 mg L-1 kinetin, pH 5.7, in the dark. After 110 days of subculture, the calli were transferred to liquid medium. Cells were kept in the dark under agitation at 110 rpm and 25 °C and subcultured every 30 days. After 90 days of culture, 20 mL aliquots of cell suspension were added to flasks containing approximately 20 mL of medium (1:1) and cultured at different wavelengths (white, green, blue, red, and blue/red) under a photoperiod of 16 h with irradiance of 50 μmol m-2 s-1) and in the absence of light. The experiment was performed in a 6 × 6 factorial design (light condition × culture time). The cell cultures showed viability throughout the entire cycle, and chlorogenic and ferulic acids, orientin, quercitrin and, in higher amounts, quercetin, were detected in the first 7 days of culture. There was an increase in superoxide dismutase and catalase and a decrease in ascorbate peroxidase after exposure to different light conditions; for phenylalanine ammonia lyase, no differences were observed. The different light conditions were not sufficient to trigger responses in the concentrations of bioactive compounds, despite the detection of increased levels of the enzymes involved in cellular homeostasis.

中文翻译:

光谱质量作为刺突茄JACQ细胞悬浮液中生物活性化合物产生的引发剂。

Solanum aculeatissimum Jacq。是巴西大部分地区的常见植物。尽管含有具有广泛药理应用的代谢物,但该植物的组织培养报道很少。这种材料大规模体外生产的可能性具有重大的生物技术潜力。因此,本研究的目的是研究光照条件对悬浮细胞生长的影响,观察生物量和生物活性化合物的产量和产量以及酶的行为。在黑暗中,在补充有1 mg L-1的2,4-D,2.5 mg L-1激动素,pH 5.7的固体培养基中培养从叶段获得的愈伤组织。继代培养110天后,将愈伤组织转移至液体培养基中。将细胞在110 rpm和25°C的搅拌下保持在黑暗中,每30天传代培养一次。培养90天后,将20 mL等分试样的细胞悬液添加到含有约20 mL培养基(1:1)的烧瓶中,并在90°C的光周期下以不同的波长(白色,绿色,蓝色,红色和蓝色/红色)进行培养。在50μmolm-2 s-1的光照下16 h,并且在没有光照的情况下。实验以6×6阶乘设计(光照条件×培养时间)进行。细胞培养物在整个周期中均显示出活力,并且在培养的前7天检测到绿原酸和阿魏酸,Orientin,槲皮素,以及大量的槲皮素。暴露于不同光照条件下,超氧化物歧化酶和过氧化氢酶增加,抗坏血酸过氧化物酶减少;对于苯丙氨酸氨裂解酶,未观察到差异。
更新日期:2020-02-10
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