Vibrio is a recognized fast-growing bacterial genus, which is considered to be attractive for the development of next-generation biotechnological workhorses. Here, three Vibrio strains FA1, FA2, and FA3, capable of growing rapidly in cost-effective media, are isolated and systematically evaluated. Genome sequencing and comparative genomic analyses are performed to reveal the underlying genetic differences between the strains and estimate their biotechnological potential. Studies of their phylogenetic tree, colinear visualization, and orthology uncover some difference in the gene content related to cell growth of the four Vibrio strains FA1, FA2, FA3, and ATCC 14048, which may explain growth superiority of the isolated strains. It is noted that there are more copies of several genes related to the DNA replication in the FA2 genome than in the other compared Vibrio strains. Furthermore, the genes responsible for amino synthesis are found, such as asD, within strains FA1 and FA2. Gene cluster cadABC, which relates to cell adaptation at acidic pH, only exists in strains FA1, FA2, and FA3. Finally, the wide spectra of substrates and genetic operability of these three isolated Vibrio strains are initially verified. This study provides excellent candidates for the development of next-generation fast-growing microbial workhorses, which may be very useful in synthetic biology.

中文翻译：

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下一代微生物主力：快速生长弧菌菌株的比较基因组分析揭示了它们的生物技术潜力。

弧菌是公认的快速增长的细菌属，被认为对下一代生物技术的发展很有吸引力。在这里，分离并系统地评估了三种可以在经济高效的培养基中快速生长的弧菌菌株FA1，FA2和FA3。进行基因组测序和比较基因组分析，以揭示菌株之间潜在的遗传差异，并估计其生物技术潜力。对它们的系统树，共线可视化和正字学的研究发现，与四种弧菌弧菌FA1，FA2，FA3和ATCC 14048的细胞生长相关的基因含量存在某些差异，这可能解释了分离株的生长优势。应当指出，与其他比较的弧菌菌株相比，FA2基因组中与DNA复制有关的几个基因的拷贝要多。此外，在菌株FA1和FA2中发现了负责氨基合成的基因，例如D。基因簇cadABC与酸性pH下的细胞适应有关，仅存在于FA1，FA2和FA3菌株中。最后，最初验证了这三个分离的弧菌菌株的底物的宽光谱和遗传可操作性。这项研究为开发下一代快速增长的微生物工作马力提供了极好的候选者，这在合成生物学中可能非常有用。仅存在于菌株FA1，FA2和FA3中。最后，最初验证了这三个分离的弧菌菌株的底物的宽光谱和遗传可操作性。这项研究为开发下一代快速增长的微生物主力提供了极好的候选者，这在合成生物学中可能非常有用。仅存在于菌株FA1，FA2和FA3中。最后，最初验证了这三个分离的弧菌菌株的底物的宽光谱和遗传可操作性。这项研究为开发下一代快速增长的微生物工作马力提供了极好的候选者，这在合成生物学中可能非常有用。