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Cancer Liquid Biopsy Using Integrated Microfluidic Exosome Analysis Platforms.
Biotechnology Journal ( IF 4.7 ) Pub Date : 2020-02-20 , DOI: 10.1002/biot.201900225
Guiying Li 1, 2 , Weiwei Tang 1 , Fang Yang 1
Affiliation  

Liquid biopsies serve as both powerful noninvasive diagnostic tools for early cancer screening and prognostic tools for monitoring cancer progression and treatment efficacy. Exosomes are promising biomarkers for liquid biopsies, since these nano-sized extracellular vesicles (EVs) enrich proteins, lipids, mRNAs, and miRNAs from cells of origin, including cancer cells. Although exosomes are abundantly present in various bodily fluids, conventional exosome isolation and detection methods that rely on benchtop equipment are time-consuming, expensive, and involve complicated non-portable procedures. As an alternative, recently developed microfluidic platforms can perform effective exosome separation and detection for liquid biopsies using a single device. Such methods offer advantages of integrity, speed, cost-efficiency, and portability over conventional benchtop and early microfluidic-based single-functional methods which can only separate or detect exosomes separately. These advances have made exosome-based point-of-care (POC) applications possible. This review outlines recent integrated microfluidic-based exosomal detection strategies to guide future development of such devices for use in liquid biopsies for early cancer screening, prognostic monitoring, and other potential POC applications.

中文翻译:

使用集成的微流体外泌体分析平台进行癌症液体活检。

液体活检既可以用作早期癌症筛查的强大无创诊断工具,也可以作为监测癌症进展和治疗效果的预后工具。外来体是用于液体活检的有前途的生物标志物,因为这些纳米级的细胞外囊泡(EVs)可从包括癌细胞在内的原始细胞中富集蛋白质,脂质,mRNA和miRNA。尽管外泌体大量存在于各种体液中,但是依靠台式设备的常规外泌体分离和检测方法既费时,昂贵,又涉及复杂的非便携式程序。作为替代方案,最近开发的微流体平台可以使用单个设备对液体活检进行有效的外泌体分离和检测。此类方法具有完整性,速度,成本效益,与传统台式机和早期基于微流体的单功能方法相比具有便携性,后者只能单独分离或检测外泌体。这些进步使基于外泌体的即时护理(POC)应用成为可能。这篇综述概述了最近基于微流体的整合体外泌体检测策略,以指导此类设备在液体活检中用于早期癌症筛查,预后监测和其他潜在POC应用的未来发展。
更新日期:2020-02-20
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