BRACHIONUS: spp. (Rotifera: Monogononta) have been introduced as ecotoxicological model-organisms that are widely distributed in aquatic environments. Among the Brachionus spp., the monogonont rotifer Brachionus koreanus has been widely used for ecology, ecotoxicology, and evolution, thus, providing the whole genome data of B. koreanus is important for further understandings of in-depth molecular mechanisms. In this study, the completed assembly and characterization of the B. koreanus genome resulted in a total length of 85.7 Mb with 14,975 annotated genes. The final number of scaffolds was 567 with an N50 value and a GC content of 1.86 Mb and 24.35 %, respectively. Based on the fully constructed genome database, a total of 24 CYPs, 23 GSTs, two SODs, and a single CAT genes were identified and analyzed antioxidant activities (CAT, SOD, and GST), and transcriptional regulation of the entire CYPs, GSTs, SODs, and CAT in response to 2-ethyl-phenanthrene (2-ethyl-PHE) and piperonyl butoxide (PBO), to demonstrate the usefulness of the whole genome library of B. koreanus in response xenobiotic-induced oxidative stress. The assembled B. koreanus genome will provide a better understanding on the molecular ecotoxicology in the view of molecular mechanisms underlying toxicological responses, particularly on xenobiotic detoxification processes in the rotifer B. koreanus.

中文翻译：

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海洋轮虫Brachionus koreanus的基因组揭示了对2-乙基菲和胡椒基丁醚的抗氧化防御系统。

腕足类 （Rotifera：Monogononta）已经作为生态毒理学模型生物被引入，并在水生环境中广泛分布。在Brachionus spp。中，单子叶轮虫Brachionus koreanus已被广泛用于生态学，生态毒理学和进化，因此，提供韩国B. koreanus的全基因组数据对于深入了解深入的分子机制具有重要意义。在这项研究中，朝鲜双歧杆菌基因组的完整组装和表征导致全长85.7 Mb，其中包含14,975个带注释的基因。最终的支架数为567，N50值和GC含量分别为1.86 Mb和24.35％。基于完全构建的基因组数据库，共鉴定了24个CYP，23个GST，两个SOD和一个CAT基因，并分析了其抗氧化活性（CAT，SOD和GST），CYP，GST，SOD和CAT的转录和转录调控，以响应2-乙基菲（2-乙基-PHE）和胡椒基丁醚（PBO），以证明朝鲜双歧杆菌的整个基因组文库在异种生物诱导的氧化应激反应。从潜在的毒理学反应的分子机制，尤其是轮虫B. koreanus的异种生物解毒过程的角度来看，组装的B. koreanus基因组将提供对分子生态毒理学的更好理解。