Super-resolution microscopy provides diffraction-unlimited optical access to the intricate morphology of neurons in living brain tissue, resolving their finest structural details, which are critical for neuronal function. However, as existing image analysis software tools have been developed for diffraction-limited images, they are generally not well suited for quantifying nanoscale structures like dendritic spines. We present SpineJ, a semi-automatic ImageJ plugin that is specifically designed for this purpose. SpineJ offers an intuitive and user-friendly graphical user interface, facilitating fast, accurate, and unbiased analysis of spine morphology.

中文翻译：

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SpineJ：用于定量分析纳米级脊柱形态的软件工具

超分辨率显微镜提供对活脑组织中神经元复杂形态的无限衍射光学访问，解析它们最精细的结构细节，这对神经元功能至关重要。然而，由于现有的图像分析软件工具是针对衍射极限图像开发的，它们通常不太适合量化纳米级结构，如树突棘。我们展示了 SpineJ，这是一个专门为此目的而设计的半自动 ImageJ 插件。SpineJ 提供直观且用户友好的图形用户界面，有助于对脊柱形态进行快速、准确和无偏见的分析。