Abstract Stacked genetically modified organisms (GMOs) are gaining popularity for their ability to enhance production efficiency and improve functional properties. Due to different labeling requirements across many countries, the detection and quantification of stacked GMO content is an important concern. Although existing methods can detect pure stacked GMOs, they are considerably less efficient at identifying material that contains the stacked GMO and its single-trait “parent” GM lines. Immunohistochemistry possesses superior specificity and sensitivity in detecting the target, especially proteins. As a result, a novel quantitative technique to detect stacked GMO was developed. Two fluorescent antibodies hybridized with two specific proteins, which were expressed by the single-trait “parents” separately, were employed. By observing whether both of the two fluorescent signals existed in the same cell, the stacked GMO could be easily detected. By calculating the fluorescent signals, quantification could be determined. This method reduces errors and the non-specificity caused by PCR-based methods, and is widely applicable in the quantitative and qualitative detection of stacked GMOs. In sum, it represents a new method for high throughput detection.

中文翻译：

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一种利用荧光免疫组织化学检测堆叠转基因植物的新定量技术

摘要 堆积的转基因生物 (GMO) 因其提高生产效率和改善功能特性的能力而越来越受欢迎。由于许多国家/地区的标签要求不同，因此检测和量化堆积的 GMO 含量是一个重要问题。尽管现有方法可以检测纯堆叠转基因生物，但它们在识别含有堆叠转基因生物及其单性状“亲本”转基因品系的材料方面效率低得多。免疫组织化学在检测靶标，尤其是蛋白质方面具有卓越的特异性和灵敏度。因此，开发了一种检测堆叠转基因生物的新型定量技术。两种荧光抗体与两种特定蛋白质杂交，分别由单性状“父母”表达。通过观察两种荧光信号是否都存在于同一个细胞中，可以很容易地检测到堆叠的转基因生物。通过计算荧光信号，可以确定定量。该方法减少了基于PCR方法的错误和非特异性，广泛适用于堆叠转基因生物的定量和定性检测。总之，它代表了一种新的高通量检测方法。