Abstract The aim of this research is to study a sustainable method to recover whole proteins from microalgae Chlorella vulgaris FSP-E using osmotic shock through liquid biphasic flotation (LBF) system. Osmotic shock induces the change in solute concentration around the cell, which results in permeation thus resulting in disruption of cell wall. Various parameters affecting the protein recovery including types of salt, concentrations of salt, types of alcohol, concentrations of alcohol, air flow rates, osmotic shock period and microalgae biomass concentration were studied. The feasibility of recycling and scaling up were also studied. The optimum parameters according to this study was shown to give a protein recovery of 92.98 % with a separation efficiency of 64.91 %, partition coefficient of 1.47 and a volume ratio of 9. Without osmotic shock, these values were obtained to be 84.84 %, 69.68 %, 1.89 and 2.96 respectively. A 5x scale up of the osmotic shock process gives the protein recovery of 91.23 % with a separation efficiency of 82.46 %, partition coefficient of 3.47 and a volume ratio of 1.50. Hence, this novel approach shows a promising design in terms of protein recovery and sustainability in a large scale.

中文翻译：

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渗透冲击辅助液体双相系统整合微藻小球藻蛋白质提取

摘要 本研究的目的是研究一种可持续的方法，通过液体双相浮选 (LBF) 系统使用渗透休克从微藻小球藻 FSP-E 中回收完整蛋白质。渗透休克引起细胞周围溶质浓度的变化，导致渗透，从而导致细胞壁破裂。研究了影响蛋白质回收的各种参数，包括盐的类型、盐的浓度、酒精的类型、酒精的浓度、空气流速、渗透冲击期和微藻生物量浓度。还研究了回收和扩大规模的可行性。根据这项研究，最佳参数显示蛋白质回收率为 92.98%，分离效率为 64.91%，分配系数为 1.47，体积比为 9。没有渗透冲击，获得的这些值分别为 84.84%、69.68%、1.89 和 2.96。渗透冲击过程放大 5 倍，蛋白质回收率为 91.23%，分离效率为 82.46%，分配系数为 3.47，体积比为 1.50。因此，这种新方法在大规模蛋白质回收和可持续性方面显示出有前途的设计。