This study investigated lipid alterations in muscle tissues [gastrocnemius (Gas) and soleus (Sol)] of mice under different diet programs (weight gain, weight maintenance, weight regain, and controls) by nanoflow ultrahigh pressure liquid chromatography-electrospray ionization-tandem mass spectrometry. Since overloaded lipids in the skeletal muscle tissues by excessive fat accumulation are related to insulin resistance leading to type II diabetes mellitus, analysis of lipid alteration in muscle tissues with respect to high-fat diet (HFD) is important to understand obesity related diseases. A total of 345 individual lipid species were identified with their molecular structures, and 184 lipids were quantified by selected reaction monitoring method. Most triacylglycerol (TG) and phosphatidylethanolamine (PE) species displayed a significant (>2-fold, p < 0.01) increase in both the Gas and Sol and to a larger degree in the Gas. However, lipid classes involved in insulin resistance and anti-inflammatory response, including lysophosphatidylcholine (18:0), diacylglycerol (16:0_18:1, 16:0_18:2, and 18:1_18:1), ceramide (d18:1/24:0 and d18:1/24:1), and phosphatidylinositol (18:0/20:4), showed a significant accumulation in the Sol exclusively after HFD treatment. In addition, the lipid profiles were not significantly altered in mice that were fed HFD only for the last 4 weeks (weight gain group), suggesting that consuming HFD in the younger age period can be more effective in the Gas. This study reveals that lipid classes related to insulin resistance accumulated more in the Sol than in the Gas following HFD treatment and the weight regain program perturbed lipid profiles of the Sol to a greater extent than that by the other diet programs, confirming that the Sol tissue is more influenced by HFD than Gas.

中文翻译：

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通过使用纳流超高效液相色谱-串联质谱法喂养高脂饮食，体重恢复后，小鼠骨骼肌组织中的脂质变化。

这项研究通过纳流超高压液相色谱-电喷雾电离串联质谱法研究了不同饮食计划（体重增加，体重维持，体重恢复和控制）下小鼠肌肉组织[腓肠肌和比目鱼肌（Sol）]中脂质的变化光谱法。由于过量脂肪积聚导致骨骼肌组织中脂质超负荷与导致II型糖尿病的胰岛素抵抗有关，因此针对高脂饮食（HFD）进行肌肉组织脂质变化分析对于了解与肥胖相关的疾病非常重要。通过分子结构鉴定出总共345种脂质，并通过选择的反应监测方法对184种脂质进行了定量。大多数三酰基甘油（TG）和磷脂酰乙醇胺（PE）种类显示出显着的（> 2倍，p <0.01）在气体和溶胶中都增加，并且在气体中增加程度更大。但是，涉及胰岛素抵抗和抗炎反应的脂质类别包括溶血磷脂酰胆碱（18：0），二酰基甘油（16：0_18：1、16：0_18：2和18：1_18：1），神经酰胺（d18：1 / 24：0和d18：1/24：1）和磷脂酰肌醇（18：0/20：4）仅在HFD处理后显示出在Sol中的大量积累。此外，仅在最近4周喂HFD的小鼠（体重增加组）的脂质分布没有明显改变，这表明在年轻时期食用HFD可以更有效地利用Gas。