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Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs.
BMC Microbiology ( IF 4.2 ) Pub Date : 2020-02-07 , DOI: 10.1186/s12866-020-1716-z
Shaobo Yu 1 , Guangyu Zhang 1 , Zhibo Liu 1 , Peng Wu 1 , Zhongtang Yu 2 , Jiakun Wang 1
Affiliation  

BACKGROUND Many recent studies have gravitated towards manipulating the gastrointestinal (GI) microbiome of livestock to improve host nutrition and health using dietary interventions. Few studies, however, have evaluated if inoculation with rumen fluid could effectively reprogram the development of GI microbiota. We hypothesized that inoculation with rumen fluid at an early age could modulate the development of GI microbiota because of its low colonization resistance. RESULTS In this study, we tested the above hypothesis using young lambs as a model. Young lambs were orally inoculated repeatedly (four times before or twice during gradual weaning) with the rumen fluid collected from adult sheep. The oral inoculation did not significantly affect starter intake, growth performance, or ruminal fermentation. Based on sequencing analysis of 16S rRNA gene amplicons, however, the inoculation (both before and during weaning) affected the assemblage of the rumen microbiota, increasing or enabling some bacterial taxa to colonize the rumen. These included operational taxonomic units (OTUs) belonging to Moryella, Acetitomaculum, Tyzzerella 4, Succiniclasticum, Prevotella 1, Lachnospiraceae, Christensenellaceae R-7 group, Family XIII AD3011, and Bacteroidales S24-7 corresponding to inoculation before weaning; and OTUs belonging to Succiniclasticum, Prevotellaceae UCG-003, Erysipelotrichaceae UCG-004, Prevotella 1, Bacteroidales S24-7 gut group uncultured bacterium, and candidate Family XIII AD3011 corresponding to inoculation during weaning. Compared to the inoculation during weaning, the inoculation before weaning resulted in more co-occurrences of OTUs that were exclusively predominant in the inoculum. However, inoculation during weaning appeared to have more impacts on the colonic microbiota than the inoculation before weaning. Considerable successions in the microbial colonization of the GI tracts accompanied the transition from liquid feed to solid feed during weaning. CONCLUSIONS Repeated rumen fluid inoculation during early life can modulate the establishment of the microbiota in both the rumen and the colon and co-occurrence of some bacteria. Oral inoculation with rumen microbiota may be a useful approach to redirect the development of the microbiota in both the rumen and colon.

中文翻译:

断奶前或断奶期间反复用新鲜瘤胃液接种可调节微生物群组成以及羔羊瘤胃和结肠的共存。

背景技术许多最近的研究倾向于使用饮食干预来操纵牲畜的胃肠(GI)微生物组以改善宿主营养和健康。但是,很少有研究评估过用瘤胃液接种是否可以有效地重新编程胃肠道微生物群的发育。我们假设早期接种瘤胃液可以调节胃肠道微生物群的发育,因为它的定植抗性很低。结果在这项研究中,我们使用幼小羔羊作为模型检验了上述假设。用从成年绵羊收集的瘤胃液反复口服幼羊羔(逐渐断奶前四次或两次逐渐断奶期间两次)。口服接种并未显着影响发酵剂的摄入,生长性能或瘤胃发酵。但是,基于对16S rRNA基因扩增子的测序分析,接种(断奶前和断奶期间)会影响瘤胃微生物群的组装,从而增加或使某些细菌类群在瘤胃中定殖。其中包括属于Moryella,Acetitomaculum,Tyzzerella 4,Succiniclasticum,Prevotella 1,Lachnospiraceae,Christensenellaceae R-7组,XIII家族AD3011和细菌杆菌S24-7的操作生物分类单位(OTU),分别对应于断奶前的接种;属于琥珀酸杆菌属,普乐藻科UCG-003,金毛菊科UCG-004,普雷沃氏菌1,细菌杆菌S24-7肠道组未培养细菌的OTU和对应于断奶期间接种的候选家族XIII AD3011。与断奶期间的接种相比,断奶前的接种导致更多的OTU同时出现,而OTU在接种物中占绝对优势。但是,断奶期间的接种似乎比断奶前的接种对结肠微生物群的影响更大。在断奶期间,胃肠道的微生物定殖有相当大的顺序伴随着从液体饲料到固体饲料的过渡。结论早期重复接种瘤胃液可以调节瘤胃和结肠中微生物群的建立以及某些细菌的共现。瘤胃微生物菌群的口服接种可能是重定向瘤胃和结肠中菌群发育的有用方法。断奶期间接种对结肠菌群的影响似乎比断奶前接种对结肠菌群的影响更大。在断奶期间,胃肠道的微生物定殖有相当大的顺序伴随着从液体饲料到固体饲料的过渡。结论早期重复接种瘤胃液可以调节瘤胃和结肠中微生物群的建立以及某些细菌的共现。瘤胃微生物菌群的口服接种可能是重定向瘤胃和结肠中菌群发育的有用方法。断奶期间接种对结肠菌群的影响似乎比断奶前接种对结肠菌群的影响更大。在断奶期间,胃肠道的微生物定殖有相当大的顺序伴随着从液体饲料到固体饲料的过渡。结论早期重复接种瘤胃液可以调节瘤胃和结肠中微生物群的建立以及某些细菌的共现。瘤胃微生物菌群的口服接种可能是重定向瘤胃和结肠中菌群发育的有用方法。结论早期重复接种瘤胃液可以调节瘤胃和结肠中微生物群的建立以及某些细菌的共现。瘤胃微生物菌群的口服接种可能是重定向瘤胃和结肠中菌群发育的有用方法。结论早期重复接种瘤胃液可以调节瘤胃和结肠中微生物群的建立以及某些细菌的共现。瘤胃微生物菌群的口服接种可能是重定向瘤胃和结肠中菌群发育的有用方法。
更新日期:2020-02-07
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