The measure of hemolysis in humans is clinically important. Here we describe methods using a gas chromatograph equipped with a reduction gas detector to detect the human analyte carbon monoxide (CO) that were developed for the extreme environment of the International Space Station. These methods can be adapted to in-hospital use for clinical care with characteristics that may surpass existing measures of hemolysis. We demonstrate improved performance over previous-generation methods in terms of reproducibility, accuracy, control for physical and intervening factors to quantitatively assess hemolysis rates at unprecedented levels. The presented measure of hemolysis using CO elimination is based on a different physiological approach that can complement and augment existing detection tools. In addition to their suitability for extreme environments, the methods present distinctive advantages over existing markers for the diagnosis, monitoring and response to treatment of hemolytic anemia. These methods have the potential to fulfill a wide range of research and clinical applications.

中文翻译：

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使用内源性一氧化碳消除技术在临床和极端环境中测量人体溶血。

人体溶血的措施在临床上很重要。在这里，我们介绍了使用配备有还原气体检测器的气相色谱仪检测人类分析物一氧化碳（CO）的方法，这些方法是为国际空间站的极端环境开发的。这些方法可以适应医院内的临床护理用途，其特征可能会超过现有的溶血措施。我们证明了在重现性，准确性，对物理和干预因素的控制方面能够以前所未有的水平定量评估溶血速率，从而比上一代方法具有更高的性能。提出的使用一氧化碳消除进行溶血的措施是基于不同的生理方法，可以补充和增强现有的检测工具。除了适用于极端环境外，与溶血性贫血的诊断，监测和反应相比，该方法具有优于现有标记物的独特优势。这些方法具有实现广泛研究和临床应用的潜力。