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Application of antagonist Bacillus amyloliquefaciens NCPSJ7 against Botrytis cinerea in postharvest Red Globe grapes.
Food Science & Nutrition ( IF 3.9 ) Pub Date : 2020-02-07 , DOI: 10.1002/fsn3.1434
Qingxin Zhou 1, 2 , Maorun Fu 3 , Minhui Xu 3 , Xiangyan Chen 1 , Jiying Qiu 1 , Fengli Wang 1, 2 , Ran Yan 3 , Junhua Wang 1 , Shuangzhi Zhao 1 , Xue Xin 1 , Leilei Chen 1
Affiliation  

We investigated the effects and possible mechanisms of Bacillus amyloliquefaciens NCPSJ7 against the gray mold caused by Botrytis cinerea in the postharvest Red Globe grapes. The disease incidence, lesion diameter, decay index, and some resistance‐related enzymes were evaluated. The antioxidant capacity of grape treated with 1 × 104 CFU/ml B. cinerea alone and combined with 1 × 107 CFU/ml NCPSJ7 was also determined. The results showed that NCPSJ7 + B. cinerea reduced the disease incidence, lesion diameter, and decay index of postharvest grapes and enhanced the activities of polyphenol oxidase, peroxidase, chitinase, and β‐1,3‐glucanase during different storage periods. Furthermore, the oxidative resistance, demonstrated by an escalating trend in the total phenolic content, DPPH free radical clearance rate, reducing power, and superoxide anion clearance rate after lesion presence, was improved. However, NCPSJ7 showed an inhibitory effect on gray mold, but resulted in the reduced antioxidant capacity in the grapes.

中文翻译:

拮抗解淀粉芽孢杆菌NCPSJ7对灰葡萄孢的应用在收获后的红地球葡萄中。

我们研究了解淀粉芽孢杆菌NCPSJ7对红球葡萄收获后灰葡萄孢引起的灰霉病的影响及其可能的机制。评估了疾病的发病率,病灶直径,衰变指数以及一些与抵抗有关的酶。 还测定了单独用1×10 4  CFU / ml灰质芽孢杆菌和1×10 7 CFU / ml NCPSJ7处理的葡萄的抗氧化能力。结果表明,NCPSJ7 +  B. cinerea降低了不同贮藏期葡萄收获后的疾病发生率,病斑直径和腐烂指数,并增强了多酚氧化酶,过氧化物酶,几丁质酶和β-1,3-葡聚糖酶的活性。此外,由总酚含量,DPPH自由基清除率,还原能力和病变存在后超氧化物阴离子清除率的升高趋势证明,其抗氧化性得到了改善。但是,NCPSJ7对灰霉病有抑制作用,但导致葡萄中抗氧化能力下降。
更新日期:2020-02-07
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