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Overexpression of SAPCD2 correlates with proliferation and invasion of colorectal carcinoma cells.
Cancer Cell International ( IF 5.8 ) Pub Date : 2020-02-06 , DOI: 10.1186/s12935-020-1121-6
Yage Luo 1, 2 , Lili Wang 1 , Wenwen Ran 1 , Guangqi Li 1 , Yujing Xiao 1 , Xiaonan Wang 1 , Han Zhao 1 , Xiaoming Xing 1
Affiliation  

Background Suppressor anaphase-promoting complex domain containing 2 (SAPCD2) is a novel gene playing important roles in the initiation, invasion, and metastasis of several malignancies. However, its role in colorectal carcinoma (CRC) still remains unclear. Method In this study, we investigated the expression and biological function of SAPCD2 in CRC. Immunohistochemistry (IHC) for SAPCD2 was performed in 410 pairs of CRC specimens and corresponding normal epithelial tissues, and in 50 adenoma tissues. Clinical pathological factors were analyzed in relation to the expression of SAPCD2. The biological functions of SAPCD2 in CRC cells and its effect on cell cycle were investigated in vitro and in vivo through gain/loss-of-function approaches. Results IHC showed that SAPCD2 expression was significantly higher in CRC tissues compared to adenoma and normal epithelium tissues and was correlated with tumor location (p = 0.018). SAPCD2 significantly promoted cell proliferation, migration, and invasion both in vitro and in vivo (p < 0.05). In addition, SAPCD2 knockdown in CRC cells was associated with reduced G1/S transition, while overexpression caused G2/M phase arrest (p < 0.05). Conclusions In sum, SAPCD2 is overexpressed in CRC tissues and plays a critical role in CRC progression. Therefore, it might represent a promising therapeutic target for CRC treatment.

中文翻译:

SAPCD2的过表达与结直肠癌细胞的增殖和侵袭相关。

背景抑制后期促进复合结构域 2 (SAPCD2) 是一种新基因,在多种恶性肿瘤的发生、侵袭和转移中起重要作用。然而,其在结直肠癌(CRC)中的作用仍不清楚。方法在本研究中,我们研究了SAPCD2在CRC中的表达和生物学功能。在 410 对 CRC 标本和相应的正常上皮组织以及 50 个腺瘤组织中进行了 SAPCD2 的免疫组织化学 (IHC)。分析与SAPCD2表达相关的临床病理因素。SAPCD2 在 CRC 细胞中的生物学功能及其对细胞周期的影响通过获得/丧失功能的方法在体外和体内进行了研究。结果 IHC 显示,与腺瘤和正常上皮组织相比,CRC 组织中 SAPCD2 的表达显着更高,并且与肿瘤位置相关 (p = 0.018)。SAPCD2 在体外和体内均显着促进细胞增殖、迁移和侵袭(p < 0.05)。此外,CRC 细胞中的 SAPCD2 敲低与 G1/S 转换减少有关,而过表达导致 G2/M 期停滞(p < 0.05)。结论 总之,SAPCD2 在 CRC 组织中过度表达,在 CRC 进展中起关键作用。因此,它可能代表了CRC治疗的有希望的治疗靶点。CRC 细胞中的 SAPCD2 敲低与 G1/S 转换减少有关,而过表达导致 G2/M 期停滞(p < 0.05)。结论 总之,SAPCD2 在 CRC 组织中过度表达,在 CRC 进展中起关键作用。因此,它可能代表了CRC治疗的有希望的治疗靶点。CRC 细胞中的 SAPCD2 敲低与 G1/S 转换减少有关,而过表达导致 G2/M 期停滞(p < 0.05)。结论 总之,SAPCD2 在 CRC 组织中过度表达,在 CRC 进展中起关键作用。因此,它可能代表了CRC治疗的有希望的治疗靶点。
更新日期:2020-02-07
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