Ticks Acari:Ixodida transmit a greater variety of pathogens than any other blood-feeding group of arthropods. While numerous microbes have been identified inhabiting Australian Ixodidae, some of which are related to globally important tick-borne pathogens, little is known about the bacterial communities within ticks collected from Australian wildlife. In this study, 1,019 ticks were identified on 221 hosts spanning 27 wildlife species. Next-generation sequencing was used to amplify the V1-2 hypervariable region of the bacterial 16S rRNA gene from 238 ticks; Amblyomma triguttatum (n = 6), Bothriocroton auruginans (n = 11), Bothriocroton concolor (n = 20), Haemaphysalis bancrofti (n = 10), Haemaphysalis bremneri (n = 4), Haemaphysalis humerosa (n = 13), Haemaphysalis longicornis (n = 4), Ixodes antechini (n = 29), Ixodes australiensis (n = 26), Ixodes fecialis (n = 13), Ixodes holocyclus (n = 37), Ixodes myrmecobii (n = 1), Ixodes ornithorhynchi (n = 10), Ixodes tasmani (n = 51) and Ixodes trichosuri (n = 3). After bioinformatic analyses, over 14 million assigned bacterial sequences revealed the presence of recently described bacteria ‘Candidatus Borrelia tachyglossi’, ‘Candidatus Neoehrlichia australis’, ‘Candidatus Neoehrlichia arcana’ and ‘Candidatus Ehrlichia ornithorhynchi’. Furthermore, three novel Anaplasmataceae species were identified in the present study including; a Neoehrlichia sp. in I. australiensis and I. fecialis collected from quenda (Isoodon fusciventer) (Western Australia), an Anaplasma sp. from one B. concolor from echidna (Tachyglossus aculeatus) (New South Wales), and an Ehrlichia sp. from a single I. fecialis parasitising a quenda (WA). This study highlights the diversity of bacterial genera harboured within wildlife ticks, which may prove to be of medical and/or veterinary importance in the future.

壁虱Acari：Ixodida传播的病原体比任何其他采血节肢动物都要多。尽管已经发现有许多微生物栖息在澳大利亚I科中，其中有些与全球重要的tick传播病原体有关，但对从澳大利亚野生动物采集的tick中的细菌群落知之甚少。在这项研究中，在跨越27种野生动物物种的221个寄主中鉴定出1,019个tick。下一代测序用于从238个刻度中扩增出细菌16S rRNA基因的V1-2高变区。花蜱triguttatum（N = 6），Bothriocroton auruginans（N = 11），Bothriocroton长臂猿（N = 20），血蜱氏丝虫（N = 10），长角血bremneri（N = 4），血蜱humerosa（N = 13） ，长角血蜱（N = 4），硬蜱antechini（N = 29），硬蜱australiensis（N = 26），硬蜱fecialis（N = 13），硬蜱holocyclus（正= 37），Myxecobii虫（ n  = 1），ornithorhynchi虫（n = 10），塔斯马尼虫（n = 51）和trichosuri虫（n = 3）。经过生物信息学分析，超过1400万个指定细菌序列揭示了最近描述的细菌' Candidatus Borrelia tachyglossi'，' Candidatus Neoehrlichia australis'，' CandidatusNeoehrlichia arcana”和“ Candidatus Ehrlichia ornithorhynchi”。此外，在本研究中鉴定出三种新颖的无形科：新细菌属 于I. australiensis和I. fecialis取自Anaplasma sp。的昆达（Isoodon fusciventer）（西澳大利亚州）。从一个B.长臂猿从针鼹（Tachyglossus叶树）（新南威尔士州）和埃立克体属。来自单个I. fecialis寄生昆达（WA）。这项研究强调了野生动物壁虱中所含细菌属的多样性，这在将来可能具有医学和/或兽医的重要性。