A simple and reliable fluorescence sensing strategy, which depends on the strong fluorescence emission of nitrogen-doped carbon quantum dots (N-CQDs) and efficient catalytic oxidation of tyrosinase (Tyr), was demonstrated for the sensitive detection of dopamine (DA) and alpha lipoic acid (ALA). Under optimized conditions, the fluorescence intensity of N-CQDs was easily quenched by dopamine oxidation product dopaquinone with the addition of Tyr. When ALA was introduced into the sensing system, ALA could inhibit and reduce the oxidation process of DA, resulting in the fluorescence recovery of N-CQDs. This sensing platform showed a sensitive relationship to the DA and ALA concentrations within certain ranges of 0.05–15 and 0.5−55 μM, and low detection limits of 0.035 and 0.39 μM, respectively. Given the above mechanisms, the proposed biosensor was utilized for the detection of DA and ALA in real samples with satisfactory results. Moreover, we successfully measured ALA through spectrometry, which is expected to provide valuable information in medical and disease diagnosis.

为了灵敏地检测多巴胺（DA）和α，证明了一种简单而可靠的荧光传感策略，该策略依赖于氮掺杂碳量子点（N-CQDs）的强荧光发射和酪氨酸酶（Tyr）的有效催化氧化。硫辛酸（ALA）。在优化的条件下，通过添加Tyr可以通过多巴胺氧化产物多巴醌轻松淬灭N-CQD的荧光强度。将ALA引入传感系统后，ALA可以抑制和减少DA的氧化过程，从而导致N-CQD的荧光恢复。该感测平台在0.05-15和0.5-55μM的某些范围内以及对0.035和0.39μM的低检测限之间显示出对DA和ALA浓度的敏感关系。鉴于以上机制，提出的生物传感器用于真实样品中DA和ALA的检测，结果令人满意。此外，我们通过光谱法成功地测量了ALA，有望为医学和疾病诊断提供有价值的信息。