The go-or-grow hypothesis states that adherent cells undergo reversible phenotype switching between migratory and proliferative states, with cells in the migratory state being more motile than cells in the proliferative state. Here, we examine go-or-grow in two-dimensional in vitro assays using melanoma cells with fluorescent cell-cycle indicators and cell-cycle-inhibiting drugs. We analyze the experimental data using single-cell tracking to calculate mean diffusivities and compare motility between cells in different cell-cycle phases and in cell-cycle arrest. Unequivocally, our analysis does not support the go-or-grow hypothesis. We present clear evidence that cell motility is independent of the cell-cycle phase and that nonproliferative arrested cells have the same motility as cycling cells.

中文翻译：

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使用荧光细胞周期指示剂和细胞周期抑制药物检查继续或成长

go-or-grow 假说指出贴壁细胞在迁移和增殖状态之间经历可逆的表型转换，迁移状态的细胞比增殖状态的细胞更有活力。在这里，我们使用具有荧光细胞周期指示剂和细胞周期抑制药物的黑色素瘤细胞，在二维体外测定中检查去或生长。我们使用单细胞追踪分析实验数据以计算平均扩散率并比较不同细胞周期阶段和细胞周期停滞的细胞之间的运动性。毫不含糊地，我们的分析不支持继续发展或增长的假设。我们提供了明确的证据，表明细胞运动与细胞周期阶段无关，并且非增殖停滞细胞与循环细胞具有相同的运动。