The TCP family genes have been under selection during domestication in maize and related andropogoneae crops. They encode plant-specific transcription factors involved in growth and development, especially in shaping the plant morphology and architecture. Sugarcane (Saccharum spp.) is the most productive in harvesting tonnage and 5th economically valuable crops worldwide for supporting world’s sugar and fuel ethanol production. Based on recently published sugarcane genome, we performed a genome-wide analysis of this gene family in the sugarcane genome and identified 22 TCP genes (SsTCPs), with 1–4 alleles each. They distributed across 28 chromosomes of S. spontaneum. Phylogenetic analysis showed that all 22 SsTCP genes can be classifed into two major groups: class I and class II. All 22 groups of SsTCPs showed species-specific clustering with TCPs of sorghum which indicate close relationship between sorghum and Saccharum. Structural organization of SsTCP genes showed that 37 SsTCPs are intronless and of the 22 SsTCPs with introns exist in coding region, which are different with TCPs of sorghum and wheat that located in UTR region. Expression study showed that transcripts of class I SsTCPs were more abundant than transcripts of class II SsTCPs. Moreover, the expression of SsTCP5–4, SsTCP6–2, SsTCP8–1, SsTCP12, SsTCP13, SsTCP15–1, SsTCP17–1 and SsTCP17–6 displayed significant change after plant hormones treatments, which suggest their function related to plant hormones. Cis-element analysis of SsTCPs’s promoter suggests that subfunctionalization may have occurred for homoeologous genes. Taken together, our analysis of TCPs in S. spontaneum provide a good starting for further studies to elucidate their specific function in sugarcane.

中文翻译：

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自发性糖酵母TCP基因的鉴定与表达分析

在TCP玉米驯化过程中基因家族一直在选择和相关的须芒草族作物。它们编码与生长和发育有关的植物特异性转录因子，特别是在塑造植物形态和结构方面。甘蔗（Saccharum spp。）是世界上产量最高的作物，是全球第五大具有经济价值的作物，可支持世界上的糖和燃料乙醇生产。基于最近发布的甘蔗基因组，我们对该甘蔗基因组中的该基因家族进行了全基因组分析，并鉴定出22个TCP基因（SsTCPs），每个具有1-4个等位基因。它们分布在自发链球菌28条染色体上。系统发育分析表明，所有22个SsTCP基因可以分为两大类：I类和II类。全部22组SsTCPs表现出与物种特异性的聚类技术合作计划项目高粱这表明高粱之间的密切关系甘蔗。SsTCP基因的结构组织表明，在编码区中存在37个无内含子的SsTCP，在编码区中存在有22个内含子的SsTCP，这与UTR区的高粱和小麦的TCP不同。表达研究表明，Ⅰ类SsTCP的转录本比Ⅱ类SsTCP的转录本更为丰富。而且，表达植物激素处理后，SsTCP5–4，SsTCP6–2，SsTCP8–1，SsTCP12，SsTCP13，SsTCP15–1，SsTCP17–1和SsTCP17–6显示出显着变化，表明它们的功能与植物激素有关。对SsTCPs启动子的顺式分析表明，同源基因可能发生了亚功能化。综上所述，我们对自发链球菌中TCP的分析为进一步研究阐明其在甘蔗中的特定功能提供了良好的开端。