The present work focuses on three main aspects such as (1) extent of surgically managed human meniscal injury problem among the selected population (patients underwent meniscectomy), (2) viability of meniscus cells (isolated from surgical debri) at different age groups, and (3) simulation studies on model scaffolds to understand the biomechanical aspects. Thus, this study gives insights on the severity of the knee meniscus injury in the selected region and tissue engineering aspects which can in turn help in the design and development tissue-engineered construct for the regeneration of damaged tissue. Total 1025 patients (who underwent knee surgery related to meniscal injuries) data were collected from 2012 to 2015. With available additional data, main population was subdivided into two subpopulations. Retrospective and predictive statistical analyses using different statistical tools were performed for gender, age groups, causes of injury, symptoms, pre-treatment symptomatic duration, location of injury, and treatment given. Medium active persons with 23–28 BMI had shown more meniscus damage occurrences in subpopulation 2 (n = 316). 125 meniscus surgical debrises were collected and cells were isolated from the samples to analyze the cell count in each age group. This study showed that as age increases, cell viability was found to be decreased. In this study using simulation studies, the stress distribution against varying applied load was analyzed using COMSOL MULTIPHYSICS (version 4.3b). For this study, two different model scaffold systems were used such as poly(methyl methacrylate) (PMMA) and silicone. This approach can be a strong foundation for the development implantable scaffolds with biomechanical properties comparable to human meniscus.

中文翻译：

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膝关节半月板损伤：通过回顾性分析和计算机模拟对组织工程策略的见解

目前的工作侧重于三个主要方面，例如（1）选定人群（接受半月板切除术的患者）中手术处理的人类半月板损伤问题的程度，（2）不同年龄组半月板细胞（从手术碎片中分离）的活力，以及(3) 模拟研究模型支架以了解生物力学方面。因此，这项研究对选定区域和组织工程方面的膝关节半月板损伤的严重程度提供了见解，这反过来有助于设计和开发组织工程结构，以再生受损组织。从 2012 年到 2015 年共收集了 1025 名患者（接受了与半月板损伤相关的膝关节手术）数据。根据可用的额外数据，主要人群被细分为两个亚群。使用不同统计工具对性别、年龄组、损伤原因、症状、治疗前症状持续时间、损伤部位和给予的治疗进行回顾性和预测性统计分析。BMI 为 23-28 的中等活跃人群在亚群 2（n = 316）中显示出更多的半月板损伤。收集125个半月板手术碎片并从样品中分离细胞以分析每个年龄组的细胞计数。这项研究表明，随着年龄的增长，细胞活力会降低。在这项使用仿真研究的研究中，使用 COMSOL MULTIPHYSICS（4.3b 版）分析了针对不同应用载荷的应力分布。在这项研究中，使用了两种不同的模型支架系统，例如聚（甲基丙烯酸甲酯）（PMMA）和硅胶。