BackgroundDaclatasvir is a direct-acting antiviral agent against hepatitis C virus (HCV) used for the treatment of chronic HCV genotype infections of 1 and 3. Hepatitis C is an infectious liver disease caused by infection with hepatitis C virus (HCV). There are no reports found to be daclatasvir in ionic liquid-based extraction.MethodsA simple vortex assisted an environmental eco-friendly ionic liquid dispersive liquid–liquid microextraction method for determination of daclatasvir form rat serum. For the sample extraction, various green solvents, like ionic liquids, were used. The repercussion of various dispersive solvents, extractant, and disperser ratios was evaluated; non-identical ionic liquids assess the salt concentration on sample recoveries and enrichment factors were examined. Amid all the ionic liquids that were scrutinized, 1-butyl-3-methylimidazolium hexafluorophosphate was selected as the most effective ionic liquid.ResultsThe present bioassay recoveries were found to be more than 99.4% at an extractant and disperser ratio of 0.43 with an addition of 5.0% NaCl (sodium chloride) that was selected as an effective salt concentration for present extraction. Compared to protein precipitation, the enhanced detection and quantification limits attained were 0.015 μg/mL and 0.045 μg/mL, respectively. A linear relationship in the range of 0.05–10.0 μg/mL respectively with a correlation coefficient of (r2) 0.9996 was observed.ConclusionThe developed method was successfully applied to study the pharmacokinetics of daclatasvir in rat serum according to the bioanalytical method validation guidelines.

中文翻译：

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基于离子液体的涡流辅助 DLLME 和 RP-LC-PDA 方法用于大鼠血清中达卡他韦的生物测定：在药代动力学中的应用

背景Daclatasvir 是一种针对丙型肝炎病毒 (HCV) 的直接作用抗病毒剂，用于治疗慢性 HCV 基因型 1 和 3 感染。丙型肝炎是由丙型肝炎病毒 (HCV) 感染引起的传染性肝病。没有发现在基于离子液体的提取中发现达卡他韦的报道。方法采用简单涡流辅助环境友好型离子液体分散液-液微萃取法测定达卡他韦型大鼠血清。对于样品提取，使用了各种绿色溶剂，如离子液体。评估了各种分散溶剂、萃取剂和分散剂比例的影响；不同离子液体评估盐浓度对样品回收率和富集因子的影响。在所有被审查的离子液体中，选择 1-丁基-3-甲基咪唑鎓六氟磷酸盐作为最有效的离子液体。结果发现在萃取剂和分散剂的比例为 0.43 以及添加 5.0% NaCl（氯化钠）的情况下，目前的生物测定回收率超过 99.4%。选择作为当前提取的有效盐浓度。与蛋白质沉淀相比，获得的增强检测和定量限分别为 0.015 μg/mL 和 0.045 μg/mL。分别在0.05-10.0 μg/mL范围内观察到线性关系，相关系数为(r2) 0.9996。结论根据生物分析方法验证指南，该方法成功应用于大鼠血清中达卡他韦的药代动力学研究。