It is very important to rapidly detect the contamination of Enterococcus faecalis in fermented foods such as Korean Kimchi to maintain its freshness since Kimchi is exported to all over the world. However, gene sequence of E. faecalis is very similar among various Lactobacillus. So, there have been difficulties in its screening. We have designed primers based on Bile salt hydrolase gene of E. faecalis and applied them to PCR test. PCR band was identified only from E. faecalis and only from the mixture contaminated with E. faecalis. It means that the primers we designed are highly specific for distinguishing contamination of E. faecalis. It will be possible to precisely screen within 1 h, which will greatly contribute to the prevention of food poisoning and quick quarantine.

中文翻译：

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以胆汁盐水解酶基因为选择标记，从乳酸菌混合物中筛选粪肠球菌

由于朝鲜泡菜出口到世界各地，因此必须快速检测发酵食品（例如韩国泡菜）中的粪肠球菌污染，以保持其新鲜度，这一点非常重要。然而，粪肠球菌的基因序列在各种乳杆菌中非常相似。因此，对其进行筛选存在困难。我们基于粪肠球菌的胆汁盐水解酶基因设计了引物，并将其用于PCR试验。仅从粪肠球菌和仅由粪肠球菌污染的混合物中鉴定出PCR带。这意味着我们设计的引物对区分粪肠球菌具有高度特异性。可以在1小时内进行精确筛查，这将大大有助于防止食物中毒和快速隔离。