Global demand for environmentally friendly grapevine cultivation and pest control has necessitated an improved understanding of the relationship between soil properties and beneficial, naturally occurring antagonists such as entomopathogenic fungi (EPF). Sixty six soil samples were collected from 22 vineyards in the Western Cape, South Africa. The association between soil nutrient status and EPF prevalence was examined. Fungi were isolated with methods of insect baiting and selective media. In addition, fungal isolates were tested against a key grapevine pest, Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae). Pathogenicity of fungal strains against P. ficus was assessed with an immersion bioassay at a concentration of 1 × 10⁸ conidia ml^–1. Twenty-three fungal strains were isolated and correspondence analysis of data indicated a positive association between EPF (Metarhizium robertsii (Hypocreales: Clavicipitaceae) and Clonostachys rosea f. catenulata [Hypocreales: Bionectriaceae]) occurrence and optimum to high levels of soil-based macronutrients (C, N and Ca). Logistic regression revealed that K was positively correlated with M. robertsii (estimate = 0.03 ± 0.01; P < 0.05; odds ratio = 1.03). Strains of Beauvaria bassiana (Hypocreales: Cordycipitaceae) caused the highest mortalities (77.0 ± 2.0% to 87.0 ± 3.0%). This study showed that some soil nutrient properties corresponded to greater occurrence of EPF in grapevine soils.

中文翻译：

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葡萄园土壤化学特性与致病菌引起平面菌不同死亡率的关联

全球对环保葡萄种植和病虫害防治的需求已使人们对土壤特性与有益的，天然存在的拮抗剂（如昆虫病原真菌（EPF））之间的关系有了更好的了解。从南非西开普省的22个葡萄园中收集了66个土壤样品。检查了土壤养分状况与EPF发生率之间的关联。用昆虫诱饵和选择性培养基分离真菌。此外，测试了真菌分离株对关键的葡萄害虫榕球菌（Signoret）（半翅目：假球菌）的抵抗力。用浸没生物测定法以浓度为1×10 ⁸分生孢子ml ^–1的浓度评估真菌菌株对榕小球藻的致病性。。二十三个真菌菌株进行分离和数据的对应分析表明EPF之间的正关联（绿僵robertsii（肉座菌目：Clavicipitaceae）和。Clonostachys糠疹˚Fcatenulata发生与最佳高水平基于土壤的常量营养物（：[Bionectriaceae肉座菌目]） C，N和Ca）。Logistic回归分析显示，K与罗伯氏支原体呈正相关（估计值= 0.03±0.01；P  <0.05；优势比= 1.03）。的菌株Beauvaria白僵菌（肉座菌目：Cordycipitaceae）引起的最高死亡率（77.0±2.0％87.0±3.0％）。这项研究表明，某些土壤养分特性对应于葡萄土壤中EPF的发生率更高。