The red-crowned crane (Grus japonensis) is an endangered wading bird distributed in northeast Asia. The population of G. japonensis is continuing to decline owing to the loss and degradation of habitats. In order to protect G. japonensis, we developed 33 single nucleotide polymorphism (SNP) markers for this species using a genotyping-by-sequencing approach. The minor allele frequency ranged from 0.033 to 0.483, the observed heterozygosity ranged from 0.067 to 0.833, the expected heterozygosity ranged from 0.066 to 0.508, and the polymorphism information content ranged from 0.062 to 0.375. Among these SNPs, two loci showed deviations from the Hardy–Weinberg equilibrium (P < 0.05). The novel polymorphic SNPs can lay the foundation for future studies on evolution and conservation genetics of G. japonensis.

丹顶鹤（Grus japonensis）是分布在东北亚的濒危涉禽。由于生境的丧失和退化，日本粳稻的种群持续减少。为了保护日本龟，我们使用基因分型测序方法为该物种开发了33个单核苷酸多态性（SNP）标记。次要等位基因频率为0.033至0.483，观察到的杂合度为0.067至0.833，预期的杂合度为0.066至0.508，多态性信息含量为0.062至0.375。在这些SNP中，两个基因座显示出与Hardy-Weinberg平衡的偏离（P <0.05）。新的多态性SNPs可以为今后对日本G的进化和保护遗传学奠定基础。