Abstract—

This is the first study of alkali-resistant fungi under the conditions of neutral salinization at Lake Baskunchak (Astrakhan region, Russia). Fungi were isolated from the lake coast chloride soils on alkaline agar medium (pH 10.0–10.5); isolates were characterized by morphological, cultural and molecular genetic features; phylogenetic analysis and analysis of adaptation to pH and salinity were performed. It has been shown that alkali-resistant fungi at the lake coast is a polyphyletic group of ascomycetes from Sordariomycetes (nine species), Dothideomycetes (five species), Eurotiomycetes (three species), and also sterile mycelium (15 isolates). The study of the growth rates in a wide range of pH values has shown that there are both alkalotolerant and alkaliphilic fungi among the isolates. The tolerance of isolates to elevated concentrations of sodium chloride was confirmed. The discovery of obligate alkaliphiles under conditions of neutral salinization was unexpected. Obligate alkaliphilic isolates were characterized as a new species of the genus Sodiomyces as a result of phylogenetic reconstructions. In the article, we discuss the possible ecological role of alkali-resistant fungi in saline habitats.

中文翻译：

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氯化物巴斯昆恰克湖沿岸首次发现耐碱真菌

摘要-

这是在巴斯昆恰克湖（俄罗斯阿斯特拉罕地区）中性盐碱化条件下对耐碱真菌的首次研究。在碱性琼脂培养基（pH 10.0-10.5）上从湖滨氯化物土壤中分离出真菌；分离株具有形态，文化和分子遗传特征；进行了系统发育分析和对pH和盐度的适应性分析。已经表明，在湖沿岸的耐碱真菌是来自伞形菌（9种），线粒菌（5种），线形菌（3种）以及无菌菌丝体（15种）的多囊菌。对各种pH值下的生长速率的研究表明，分离株中既有耐碱性的又有碱性的真菌。确认了分离株对氯化钠浓度升高的耐受性。在中性盐化条件下发现专性亲碱是出乎意料的。专性嗜碱分离物被鉴定为一种新的属Sodiomyces系统发育重建的结果。在本文中，我们讨论了耐盐真菌在盐碱生境中可能的生态作用。