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Evaluation of in vitro alpha-glucosidase inhibitory, antimicrobial, and cytotoxic activities of secondary metabolites from the endophytic fungus, Nigrospora sphaerica, isolated from Helianthus annuus
Annals of Microbiology ( IF 3 ) Pub Date : 2019-11-13 , DOI: 10.1007/s13213-019-01523-1
Preuttiporn Supaphon , Sita Preedanon

This study aimed to evaluate alpha-glucosidase inhibition and antimicrobial activity as well as cytotoxic activity of extracts from the endophytic fungus, Nigrospora sp., isolated from leaves of Helianthus annuus, which is widely cultivated for food and used as a medicinal plant. The fungus (TSU-CS003) was identified based on internal transcribed spacer ribosomal DNA sequences and fungal biomass, and fermentation broth was subjected to extraction by solvents (hexane and ethyl acetate). All extracts were tested for their antimicrobial activity, alpha-glucosidase inhibition, and cytotoxicity activity. In addition, the active extract was analyzed by using gas chromatography mass spectrometry (GC-MS) TSU-CS003 was identified as Nigrospora sphaerica. The fermentation broth extract (BE) showed strong antimicrobial activity against Staphylococcus aureus and methicillin-resistant S. aureus (Gram-positive bacteria) with minimum inhibitory concentration (MIC) values in the range of 16–32 μg/mL and a few yeasts with MIC values ranging from 64 to 128 μg/mL, especially Talaromyces marneffei with an MIC value of 4 μg/mL. The effects of BE were observed by SEM. The results showed that this extract affected the cell morphology of T. marneffei. The half-maximal inhibitory concentration (IC50) of BE from alpha-glucosidase inhibition was recorded as 17.25 μg/mL and also showed significant cytotoxicity against A549 human cancer cell lines with an IC50 value of 22.41 μg/mL. Furthermore, BE was analyzed by using GC-MS and divided into three main compounds, including 5-pentyldihydrofuran-2(3H)-one, (Z)-methyl 4-(isobutyryloxy)but-3-enoate, and 2-phenylacetic acid. This was the first report of the endophytic fungus N. sphaerica from H. annuus. It is a potential source of active metabolites, which gave the strong antifungal activity, antioxidant activity, and cytotoxicity to A549 cancer cell lines.

中文翻译:

评价从向日葵提取的内生真菌Nigrospora sphaerica的次生代谢产物的体外α-葡萄糖苷酶抑制,抗菌和细胞毒性活性

这项研究旨在评估从向日葵叶片中分离出来的内生真菌Nigrospora sp。的提取物对α-葡萄糖苷酶的抑制和抗菌活性以及细胞毒性活性,该向日葵叶片广泛用于食品并用作药用植物。根据内部转录的间隔区核糖体DNA序列和真菌生物量鉴定真菌(TSU-CS003),然后用溶剂(己烷和乙酸乙酯)提取发酵液。测试所有提取物的抗微生物活性,α-葡萄糖苷酶抑制作用和细胞毒性活性。另外,通过使用气相色谱质谱法(GC-MS)分析活性提取物,将TSU-CS003鉴定为球形黑穗霉(Nigrospora sphaerica)。发酵液提取物(BE)对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌(革兰氏阳性细菌)表现出强大的抗菌活性,其最小抑菌浓度(MIC)值在16–32μg/ mL的范围内,一些酵母具有MIC值范围从64到128μg/ mL,特别是Marneffei Talaromyces marneffei,MIC值为4μg/ mL。通过SEM观察BE的影响。结果表明,该提取物影响T. marneffei的细胞形态。记录的来自α-葡萄糖苷酶抑制的BE的半数最大抑制浓度(IC50)为17.25μg/ mL,并且还显示出对A549人癌细胞系的显着细胞毒性,IC50值为22.41μg/ mL。此外,使用GC-MS分析了BE,并将其分为三个主要化合物,包括5-戊基二氢呋喃-2(3H)-,4-(异丁酰氧基)丁-3-烯酸酯(Z)-甲基和2-苯基乙酸。这是来自H. annuus的内生真菌N. sphaerica的首次报道。它是活性代谢产物的潜在来源,具有强大的抗真菌活性,抗氧化活性和对A549癌细胞系的细胞毒性。
更新日期:2020-04-18
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