The present study aimed to examine the effects of applying composted aquatic plants (CAP) on richness and composition of soil bacterial assemblages responsible for alkaline phosphatase production by pot experiments, as compared to the corresponding effects of inorganic chemical fertilizers (ICF). Soil bacterial assemblages were studied by metabarcoding the 16S region of ribosomal DNA as a universal genetic marker and the phoD alkaline phosphatase gene as a functional marker. The compositions of 16S and phoD assemblages were significantly different among soils applied with CAP, ICF, their mixture (MIX), and control (no application). Furthermore, the richness of phoD assemblages in soils was greater in CAP or MIX application than in ICF or control, although significant difference only existed between MIX and ICF. In conclusion, the present study illustrated that the application of composted aquatic plants affected the richness and composition of soil bacterial assemblages responsible for the production of alkaline phosphatase.

中文翻译：

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堆肥水生植物对土壤中细菌16S rDNA和碱性磷酸酶基因多样性的影响

本研究旨在通过盆栽试验研究堆肥水生植物（CAP）对负责碱性磷酸酶生产的土壤细菌群落的丰富度和组成的影响，以及无机化学肥料（ICF）的相应影响。通过对核糖体DNA的16S区作为通用遗传标记和phoD碱性磷酸酶基因作为功能标记进行metbarbarcoding，研究了土壤细菌的集合。在使用CAP，ICF，其混合物（MIX）和对照（未施用）的土壤中，16S和phoD组合物的组成明显不同。此外，phoD的丰富性尽管仅在MIX和ICF之间存在显着差异，但CAP或MIX施用的土壤中的组合物大于ICF或对照。总之，本研究表明，堆肥水生植物的应用影响了负责碱性磷酸酶生产的土壤细菌组合的丰富性和组成。