Sox2 is an embryonal stem cell Ag essential for early embryonic development, tissue homeostasis and immune regulation. In the current study, one complete Sox2 cDNA sequence was cloned from freshwater bivalve Anodonta woodiana and named AwSox2. Histological changes of testis derived from Bisphenol A (BPA) treatment were analyzed by hematoxylin and eosin staining. Expressions of AwSox2 derived from BPA, LPS and polyinosinic:polycytidylic (Poly I:C) challenge were measured by quantitative real-time PCR. The full-length cDNA of AwSox2 contained an open reading frame of 927 nucleotides bearing the typical structural features of Sox2 family. Obvious degeneration, irregular arrangement of spermatids, and clotted dead and intertwined spermatids were observed in BPA-treated groups. Administration of BPA could result in a dose-dependent up-regulation of AwSox2 expression in the male gonadal tissue of A. woodiana. In addition, expression of AwSox2 was significantly induced by LPS and Poly I:C treatment in the hepatopancreas, gill and hemocytes, compared with that of control group. These results indicated that up-regulations of AwSOx2 are closely related to apoptosis of spermatogonial stem cells derived from BPA treatment as well as enhancement of immune defense against LPS and Poly I:C challenge in A. woodiana.

Sox2 是一种胚胎干细胞 Ag，对于早期胚胎发育、组织稳态和免疫调节至关重要。本研究从淡水双壳类无齿动物Anodonta woodiana中克隆了一条完整的Sox2 cDNA序列，并将其命名为AwSox2。通过苏木精和伊红染色分析双酚 A (BPA) 处理后睾丸的组织学变化。通过定量实时 PCR 测量源自 BPA、LPS 和聚肌胞苷酸：聚胞苷酸 (Poly I:C) 攻击的 AwSox2 表达。AwSox2全长cDNA含有927个核苷酸的开放阅读框，具有Sox2家族的典型结构特征。BPA处理组精子细胞明显变性、排列不规则、凝结死亡、缠绕。BPA 的施用可能导致A. woodiana雄性性腺组织中 AwSox2 表达的剂量依赖性上调。此外，与对照组相比，LPS和Poly I:C处理在肝胰腺、鳃和血细胞中显着诱导AwSox2的表达。这些结果表明，AwSOx2 的上调与A. woodiana中 BPA 处理产生的精原干细胞凋亡以及针对 LPS 和 Poly I:C 攻击的免疫防御的增强密切相关。